Microtubules are 25nm diameter protein rods found in eukaryotic cells and are associated with a family of proteins called microtubule associated proteins (MAPs). MAPs play a crucial role in the regulation of microtubule dynamics and interactions in vivo. MAP2 isoforms are expressed only in neuronal perikarya and dendrites so appropriate antibodies are used to identify these structures in cell culture and sections.
RA22152 was made against a mixture of recombinant human projection domain sequences. It binds to the MAP2A and MAP2B isoforms but not the lower molecular weight MAP2C and MAP2D forms which lack the projection domain sequences. This antibody is of very high titre, working well at dilutions of 1:10,000.
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