Product Details
Catalog Number: RA22153
Applications: ICC, IF
Type: Rabbit IgG
Dilutions: Immunofluorescence: 1:2,000-5,000
Immunogen: KSITPETPTEIPCGDIRLNAV coupled to KLH
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (polar packs)
Format A: liquid
Species Reactivity: Human, Mouse, Rat, Bovine
Entrez: 6532
UniProt: P31645
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$275.00Add to Cart

Serotonin, also known as 5-hydroxytryptamine, is an important neurotransmitter in the CNS and has many important functions outside the CNS. Serotoninergic neuron cell bodies in the CNS are found in the raphe nucleus and send their fine beaded processes throughout the brain and spinal cord. The serotoninergic system modulates mood, perception, emotion, aggression, stress responses, sleep and appetite and contributes to learning and memory formation. The processes of serotoninergic neurons express the Na+/Cl- dependent serotonin transporter (SERT), which is responsible for uptake of serotonin from the extracellular space following synaptic release.

RA22153 was made against a peptide identical to the C-terminal 21 amino acids of human SERT and can be used to visualize serotoninergic neurons and their processes in cell culture and in sectioned material. The homologous rodent peptide differs by one conservative amino acid substitution and we have shown that this antibody works well on rodent and bovine tissues and, based on sequence conservation, is expected to be widely applicable to other mammals.

Images
Immunofluorescence

Immunofluorescent analysis of a rat brain section stained with RA22153 in green and counterstained with chicken polyclonal antibody to tyrosine hydroxylase in red. The axons of serotoninergic neurons course throughout the section but are clearly distinct from the chatacholinergic processes and cell bodies revealed with the TH antibody. The blue stain reveals DNA in cell nuclei.

Immunofluorescence

Immunofluorescent analysis of a rat brain section stained with RA22153 in green and counterstained with chicken polyclonal antibody to tyrosine hydroxylase in red. The axons of serotoninergic neurons course throughout the section but are clearly distinct from the chatacholinergic processes and cell bodies revealed with the TH antibody. The blue stain reveals DNA in cell nuclei.

Western Blot

Western blot analysis of different tissue and cell lysates using rabbit pAb to the serotonin transporter (SERT), RA22153, dilution 1:1,000 in green. Left: [1] protein standard, [2] rat brain caudate/putmen region, [3] rat brain striatum region and [4] mouse brain. Right: [5] protein standard, [6] non-transfected HEK293 cells and [7] HEK293 cells transfected with an expression construct containing a Myc-DDK tagged full length human SERT cDNA.

Western Blot

Western blot analysis of different tissue and cell lysates using rabbit pAb to the serotonin transporter (SERT), RA22153, dilution 1:1,000 in green. Left: [1] protein standard, [2] rat brain caudate/putmen region, [3] rat brain striatum region and [4] mouse brain. Right: [5] protein standard, [6] non-transfected HEK293 cells and [7] HEK293 cells transfected with an expression construct containing a Myc-DDK tagged full length human SERT cDNA.