Images
Immunofluorescence

Immunofluorescent analysis of a rat brain section stained with RA22153 in green and counterstained with chicken polyclonal antibody to tyrosine hydroxylase in red. The axons of serotoninergic neurons course throughout the section but are clearly distinct from the chatacholinergic processes and cell bodies revealed with the TH antibody. The blue stain reveals DNA in cell nuclei.

Immunofluorescence

Immunofluorescent analysis of a rat brain section stained with RA22153 in green and counterstained with chicken polyclonal antibody to tyrosine hydroxylase in red. The axons of serotoninergic neurons course throughout the section but are clearly distinct from the chatacholinergic processes and cell bodies revealed with the TH antibody. The blue stain reveals DNA in cell nuclei.

Western Blot

Western blot analysis of different tissue and cell lysates using rabbit pAb to the serotonin transporter (SERT), RA22153, dilution 1:1,000 in green. Left: [1] protein standard, [2] rat brain caudate/putmen region, [3] rat brain striatum region and [4] mouse brain. Right: [5] protein standard, [6] non-transfected HEK293 cells and [7] HEK293 cells transfected with an expression construct containing a Myc-DDK tagged full length human SERT cDNA.

Western Blot

Western blot analysis of different tissue and cell lysates using rabbit pAb to the serotonin transporter (SERT), RA22153, dilution 1:1,000 in green. Left: [1] protein standard, [2] rat brain caudate/putmen region, [3] rat brain striatum region and [4] mouse brain. Right: [5] protein standard, [6] non-transfected HEK293 cells and [7] HEK293 cells transfected with an expression construct containing a Myc-DDK tagged full length human SERT cDNA.

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