Product Details
Catalog Number: RA22155
Applications: ICC, WB, IHC, IF
Type: Rabbit IgG
Dilutions: Immunofluorescence: 1:2,000; Immunohistochemistry: 1:2,000; Western Blot: 1:10,000
Immunogen: Full length recombinant human VLP1
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (polar packs)
Format A: liquid
Species Reactivity: Human, Mouse, Rat
Entrez: 7447
UniProt: P62760
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$275.00Add to Cart

Visinin was originally isolated biochemically from chicken retina as a major protein of ~24kDa on SDS-PAGE gels. The protein belongs to the large superfamily of calmodulin and paravalbumin type proteins which function by binding Calcium ions. Calcium binding alters the conformation of these proteins and allow them to interact with other binding partners, the properties of which they may alter. Visinin-like protein 1 has four “EF hand” domains, which are negatively charged helix-turn-helix peptides which are responsible for Calcium binding. The protein is 191 amino acids in size and has a molecular weight on SDS-PAGE of 18kDa. The protein has recently been suggested to be a useful blood biomarker of Alzheimer’s disease and traumatic brain injury.

RA22155 antibody was made against full length recombinant human visinin-like protein 1, and expressed in and purified from E. Coli. It can be used to track this protein by ELISA, on western blots and in cells in culture and sections.

Images
Immunofluorescence

Immunofluorescent analysis of rat cerebellum section stained with rabbit pAb to VLP1, RA22155, dilution 1:2,000 in green, and costained with mouse mAb to calretinin in red. The blue is Hoechst staining of nuclear DNA. The VLP1 antibody reveals protein expressed in granule cells membranes and their synapses in both the granular and molecular layer of the cerebellum.

Immunofluorescence

Immunofluorescent analysis of rat cerebellum section stained with rabbit pAb to VLP1, RA22155, dilution 1:2,000 in green, and costained with mouse mAb to calretinin in red. The blue is Hoechst staining of nuclear DNA. The VLP1 antibody reveals protein expressed in granule cells membranes and their synapses in both the granular and molecular layer of the cerebellum.

Western Blot

Western blot analysis of different tissue lysates using rabbit pAb to visinin-like Protein 1 (VLP1), RA22155, dilution 1:20,000 in green: [1] protein standard (red), [2] rat brain [3] mouse brain, [4] pig hippocampus, and [5] cow cerebellum. The band at ~20kDa corresponds to the VLP1 protein.

Western Blot

Western blot analysis of different tissue lysates using rabbit pAb to visinin-like Protein 1 (VLP1), RA22155, dilution 1:20,000 in green: [1] protein standard (red), [2] rat brain [3] mouse brain, [4] pig hippocampus, and [5] cow cerebellum. The band at ~20kDa corresponds to the VLP1 protein.