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The affinity purified histochemical antibody for 5HT2A receptor was generated in rabbit against a multiple antigenic peptide of an N-terminal synthetic sequence corresponding to amino acids 22-41 of rat 5HT2A receptor. The antibody produces 4+ (maximum) immunolabeling of 5HT2A receptor at dilutions of 1/300 - 1/500 using biotin-streptavidin/HRP technique with free-floating methods on rat cortex, amygdala and hippocampus. The addition of intensifying reagents such as nickel ammonium sulfate to the chromogen solution allows further dilution to approximately 1/1,000 or more. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended. Immunolabeling is completely abolished by preadsorption with synthetic rat 5HT2A receptor (22-41). Immunolabeling of Western blot revealed a single band of approximately between 55 and 75 kDA. For Western Blot Protocol See: DOI:10.1124/jpet.111.18378. Product Highlights: |
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Product Publications
- Ramona M. Rodriguiz, Vineet Nadkarni, Christopher R. Means, Vladimir M. Pogorelov, Yi-Ting Chiu, Bryan L. Roth, and William C. Wetsel. (2021). LSD-Stimulated Behaviors in Mice Require β-Arrestin 2 but Not β-Arrestin 1. Scientific Reports, 11:17690. doi: 10.1038/s41598-021-96736-3
- Lauren F. Borkowski, Catherine L. Smith, Amy N. Keilholz, and Nicole L. Nichols. (2021). Divergent Receptor Utilization is Necessary for Phrenic Long-Term Facilitation Over the Course of Motor Neuron Loss Following CTB-SAP Intrapleural Injections. Journal of Neurophysiology. doi: 10.1152/jn.00236.2021
- Ramona M. Rodriguiz, Vineet Nadkarni, Christopher R. Means, Vladimir M. Pogorelov, Yi-Ting Chiu, Bryan L. Roth, and William C. Wetsel. (2021). Genetic Deletion of β-Arrestin 2 Modulates LSD Stimulated Behaviors in Mice. doi: 10.21203/rs.3.rs-544199/v1
- Sukanya Mozumder, Gopa Mahesh, Krishnamoorthi Srinivasan, Jayati Sengupta, and Sujoy Mukherjee. (2020). Expression and Purification of Functionally Active Serotonin 5-HT2A Receptor in Insect Cells Using Low-Titer Viral Stock. Bio-Protocol 10(15): e3704. doi: 10.21769/BioProtoc.3704
- Lauren F. Borkowski, Taylor A. Craig, Olivia E. Stricklin, Katherine A. Johnson, and Nicole L. Nichols. (2020). 5-HT2A/B Receptor Expression in the Phrenic Motor Nucleus in a Rat Model of ALS (SOD1G93A). Respiratory Physiology & Neurobiology. doi: 10.1016/j.resp.2020.103471
- Amanda E Price, Dennis J. Sholler, Sonja J. Stutz, Noelle C. Anastasio, and Kathryn A. Cunningham (2019). Endogenous Serotonin 5-HT2A and 5-HT2C Receptors Associate in the Medial Prefrontal Cortex. ACS Chem. Neurosci., DOI: 10.1021/acschemneuro.8b00669
- Vanja Dakic, Juliana Minardi Nascimento, Rafaela Costa Sartore, Renata de Moraes Maciel, Draulio B. de Araujo, Sidarta Ribeiro, Daniel Martins-de-Souza , Stevens Rehen. (2017). Short term changes in the proteome of human cerebral organoids induced by 5-methoxy-N,N-dimethyltryptamine. bioRxiv, doi: 10.1101/108159
- David A. Martin, Charles D. Nichols. (2016). Psychedelics Recruit Multiple Cellular Types and Produce Complex Transcriptional Responses Within the Brain. EBioMedicine, doi: 10.1016/j.ebiom.2016.08.049
- Xavier Viñals, Estefanía Moreno, Laurence Lanfumey, Arnau Cordomí, Antoni Pastor, Rafael de La Torre, Paola Gasperini, Gemma Navarro, Lesley A. Howell, Leonardo Pardo, Carmen Lluís, Enric I. Canela, Peter J. McCormick, Rafael Maldonado, Patricia Robledo. (2015). Cognitive Impairment Induced by Delta9-tetrahydrocannabinol Occurs through Heteromers between Cannabinoid CB1 and Serotonin 5-HT2A Receptors. PLOS Biology, doi: 10.1371/journal.pbio.1002194
- Garcia-Cabrerizo, R. & Garcia-Fuster, M.J. (2015). Chronic MDMA induces neurochemical changes in hippocampus of adolescent and young adult rats: Down-regulation of apoptotic markers. NeuroToxicology, doi: 10.1016/j.neuro.2015.06.001
- Tania Rivera-Baltanasa, Jose Manuel Olivares, Jose Ramon Martinez-Villamarin, Erin Y. Fenton, Lisa E. Kalynchuk, Hector J. Caruncho. (2014). Serotonin 2A receptor clustering in peripheral lymphocytes is altered in major depression and may be a biomarker of therapeutic efficacy. Journal of Affective Disorders. doi: 10.1016/j.jad.2014.03.011
- Noelle C. Anastasio, Scott R. Gilbertson, Marcy J. Bubar, Anton Agarkov, Sonja J. Stutz, Yowjiun Jeng, Nicole M. Bremer, Thressa D. Smith, Robert G. Fox, Sarah E. Swinford, Patricia K. Seitz, Marc N. Charendoff, John W. Craft, Jr, Fernanda M. Laezza, Cheryl S. Watson, James M. Briggs, and Kathryn A. Cunningham. (2013). Peptide Inhibitors Disrupt the Serotonin 5-HT2C Receptor Interaction with Phosphatase and Tensin Homolog to Allosterically Modulate Cellular Signaling and Behavior. The Journal of Neuroscience, 33(4):1615-1630; doi: 10.1523/JNEUROSCI.2656-12.2013
- Prem N. Yadav, Wesley K. Kroeze, Martilias S. Farrell and Bryan L. Roth. (2011). ANTAGONIST FUNCTIONAL SELECTIVITY: 5-HT2A SEROTONIN RECEPTOR ANTAGONISTS DIFFERENTIALLY REGULATE 5-HT2A RECEPTOR PROTEIN LEVEL IN VIVO. JPET, doi: 10.1124/jpet.111.18378
- Prem N Yadav, Atheir I Abbas, Martilias S Farrell, Vincent Setola, Noah Sciaky, Xi-Ping Huang, Wesley K Kroeze, LaTasha K Crawford, David A Piel, Michael J Keiser, John J Irwin, Brian K Shoichet, Evan S Deneris, Jay Gingrich, Sheryl G Beck and Bryan L Roth. (2010). The Presynaptic Component of the Serotonergic System is Required for Clozapine's Efficacy. Neuropsychopharmacology, doi: 10.1038/npp.2010.195
- Cullen L. Schmid and Laura M. Bohn. (2010). Serotonin, But Not N-Methyltryptamines, Activates the Serotonin 2A Receptor Via a β-Arrestin2/Src/Akt Signaling Complex In Vivo. J. Neurosci., 30: 13513 - 13524; doi: 10.1523/JNEUROSCI.1665-10.2010
- Ana C Magalhaes, Kevin D Holmes, Lianne B Dale, Laetitia Comps-Agrar, Dennis Lee, Prem N Yadav, Linsay Drysdale, Michael O Poulter, Bryan L Roth, Jean-Philippe Pin, Hymie Anisman & Stephen S G Ferguson. (2010). CRF receptor 1 regulates anxiety behavior via sensitization of 5-HT2 receptor signaling. Nature Neuroscience, doi: 10.1038/nn.2529
- Cullen L. Schmid, Kirsten M. Raehal, and Laura M. Bohn. (2007). Agonist-directed signaling of the serotonin 2A receptor depends on β-arrestin-2 interactions in vivo. PNAS, doi: 10.1073/pnas.0708862105
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5HT2AR staining of rat cerebral cortex.
5HT2AR staining of rat cerebral cortex.
Mouse frontal cortical neuron cultures (p1 pups, day 4 culture stained-Neuromicsantibody: 5-HT2AR at 1:100 48 hours. MAP2 Ab: 1:20,000, 24 h. Details in Schmid, Raehal, and Bohn; 2008; PNAS
Mouse frontal cortical neuron cultures (p1 pups, day 4 culture stained-Neuromicsantibody: 5-HT2AR at 1:100 48 hours. MAP2 Ab: 1:20,000, 24 h. Details in Schmid, Raehal, and Bohn; 2008; PNAS