The serotonin transporter (SERT) is a monoamine transporter, integral membrane protein that transports the neurotransmitter serotonin from synaptic spaces into presynaptic neurons. This transport of serotonin by the SERT protein terminates the action of serotonin and recycles it in a sodium-dependent manner.
The histochemical antibody for serotonin (5HT) transporter is generated in a rabbit against a synthetic peptide sequence corresponding to amino acids 579-599 of rat 5HT transporter coupled to KLH.
The antibody produces 4+ (maximum) labeling of 5HT transporter at dilutions of 1/200 - 1/400 using indirect immunofluorescence and at dilutions of 1/10,000 - 1/15,000 using biotin/streptavidin HRP technique in rat raphe nuclei, hypothalamus, cortex, and spinal cord. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended. By Western blot analysis using rat brain extracts of cortex, hypothalamus, midbrain, and hindbrain, the antibody specifically labels a single band. Immunolabeling is completely abolished by pre-adsorption with synthetic rat 5HT transporter.
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