Product Details
Catalog Number: RA24429
Applications: WB, IHC
Type: Rabbit IgG
Dilutions: IHC 1:1000-1:2000
Immunogen: synthetic peptide sequence corresponding to amino acids 17-34 of the rat 5-HT5A receptor.
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Affinity Purified
Format B: liquid
Species Reactivity: Mouse, Rat
Entrez: 3361
UniProt: P47898
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$399.00Add to Cart

The histochemical antibody for 5-HT5A receptor is generated in a rabbit against synthetic peptide sequence corresponding to amino acids 17-34 of the rat 5-HT5A receptor.

The antibody produces 4+ (maximum) labeling of 5-HT5A receptor at dilutions of 1/100 - 1/300 using both indirect immunofluorescence and biotin-streptavidin/HRP technique in rat cortex and hippocampus. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended. The antibody was characterized by immunoblotting and immunohistochemistry. Immunoblots of rat brain extracts revealed the presence of two bands at molecular weights of 41 and 47 kD. The lower weight band agrees with the calculated molecular weight based on amino acid sequence. The higher weight may represent glycosylated receptor protein. Immunohistochemical staining of rat brain correlates well with Northern blot analysis and in situ hybridization studies. Immunolabeling is completely abolished by preadsorption with synthetic rat 5-HT5A receptor (17-34). BlastP database sequence homology searches indicate that the amino acid sequence is unique to rat and mouse 5-HT5A receptor.

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