The histochemical antibody for 5-HT7 receptor is generated in a rabbit against synthetic peptide sequence corresponding to amino acids 8-23 of the rat 5-HT7 receptor.
The antibody produces 4+ (maximum) labeling of 5-HT7 receptor at dilutions of 1/100 - 1/300 using biotin-avidin/HRP technique in rat cortex and hippocampus. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended. The antibody was characterized by immunohistochemistry. Immunohistochemical staining of rat brain correlates well with Northern blot analysis, in situ hybridization and receptor autoradiography studies. Immunolabeling is completely abolished by preadsorption with synthetic rat 5-HT7 receptor (8-23). BlastP database sequence homology searches indicate that the amino acid sequence is unique to rat 5-HT7A, 5-HT7B and 5-HT7C. There is also significant sequence overlap with the mouse and human forms.
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