The histochemical antibody for 5-HT2C receptor is generated in a rabbit against synthetic peptide sequence corresponding to amino acids 439-460 of the rat 5-HT2C receptor.
The antibody produces 4+ (maximum) labeling of 5-HT2C receptor at dilutions of 1/300 - 1/500 using biotin-streptavidin/HRP technique in rat choroid plexus and hippocampus. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended. The antibody was characterized by immunohistochemistry and Western blot. Western blotting revealed a single band of approximately 70 kD. Preincubation of the antibody with an excess of the synthetic peptide blocked staining. Immunohistochemical staining of rat brain correlates well with Northern analysis, in situ hybridization and receptor autoradiography. BlastP database sequence homology searches confirmed that this sequence is unique to rat, mouse and human 5-HT2C receptors.
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