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Western Blot: OPA1 Antibody [RA25101] - Total protein from Mouse Neuro2A cells, Human HeLa cells and Rat PC12 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-OPA1 in blocking buffer, and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Western Blot: OPA1 Antibody [RA25101] - Total protein from Mouse Neuro2A cells, Human HeLa cells and Rat PC12 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-OPA1 in blocking buffer, and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Immunocytochemistry/Immunofluorescence: OPA1 Antibody [RA25101] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-OPA1 at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution.
Immunocytochemistry/Immunofluorescence: OPA1 Antibody [RA25101] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-OPA1 at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution.
Immunohistochemistry-Paraffin: OPA1 Antibody [RA25101] - IHC analysis of a formalin fixed paraffin embedded (FFPE) tissue section of mouse testes using OPA1 antibody at 1:1000 dilution. The primary antibody bound to OPA1 antigens in the tissue section was deteced using an HRP labeled secondary antibody and DAB reagent.
Immunohistochemistry-Paraffin: OPA1 Antibody [RA25101] - IHC analysis of a formalin fixed paraffin embedded (FFPE) tissue section of mouse testes using OPA1 antibody at 1:1000 dilution. The primary antibody bound to OPA1 antigens in the tissue section was deteced using an HRP labeled secondary antibody and DAB reagent.