Product Details
Catalog Number: RA25101
Applications: ICC, WB, IHC, IF
Type: Rabbit IgG
Immunogen: A synthetic peptide made to an internal region within residues 500-600 of human OPA1.
Storage: Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: liquid
Species Reactivity: Human, Mouse, Rat
Entrez: 4976
UniProt: O60313
Downloads: Datasheet (pdf)
Product Sizes
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100 µl$379.00Add to Cart

OPA1 (optic atrophy protein 1) is a dynamin-related GTPase which forms a diffusion barrier for proteins stored in mitochondrial cristae and is critical to mitochondrial fusion as well as regulation of apoptosis. Intrinsic apoptotic signals mediated proteolytic processing leads OPA1 oligomers disassembly and caspase activator cytochrome C (CYCS) release into mitochondrial intermembrane spaces. Defects in OPA1 are a cause of optic atrophy type 1 (OPA1) as well as dominant optic atrophy plus syndrome (DOA+).

Images
OPA1 Western Blot

Western Blot: OPA1 Antibody [RA25101] - Total protein from Mouse Neuro2A cells, Human HeLa cells and Rat PC12 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-OPA1 in blocking buffer, and detected with an anti-mouse HRP secondary antibody using chemiluminescence.

OPA1 Western Blot

Western Blot: OPA1 Antibody [RA25101] - Total protein from Mouse Neuro2A cells, Human HeLa cells and Rat PC12 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-OPA1 in blocking buffer, and detected with an anti-mouse HRP secondary antibody using chemiluminescence.

OPA1 Immunocytochemistry/Immunofluorescence

Immunocytochemistry/Immunofluorescence: OPA1 Antibody [RA25101] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-OPA1 at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution.

OPA1 Immunocytochemistry/Immunofluorescence

Immunocytochemistry/Immunofluorescence: OPA1 Antibody [RA25101] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-OPA1 at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution.

OPA1 Immunohistochemistry-Paraffin

Immunohistochemistry-Paraffin: OPA1 Antibody [RA25101] - IHC analysis of a formalin fixed paraffin embedded (FFPE) tissue section of mouse testes using OPA1 antibody at 1:1000 dilution. The primary antibody bound to OPA1 antigens in the tissue section was deteced using an HRP labeled secondary antibody and DAB reagent.

OPA1 Immunohistochemistry-Paraffin

Immunohistochemistry-Paraffin: OPA1 Antibody [RA25101] - IHC analysis of a formalin fixed paraffin embedded (FFPE) tissue section of mouse testes using OPA1 antibody at 1:1000 dilution. The primary antibody bound to OPA1 antigens in the tissue section was deteced using an HRP labeled secondary antibody and DAB reagent.