Product Details
Catalog Number: RA30033
Applications: WB, IHC, E
Type: Rabbit IgG
Immunogen: Synthetic peptide-Amino acids 417-425 of human SMAD3 protein. Immunogen modification: pSer423/425
Storage: Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Affinity Purified
Format B: liquid
Species Reactivity: Human, Mouse, Rat, Chicken, Bovine, Porcine
Entrez: 4088
UniProt: P84022
Downloads: Datasheet (pdf)
Product Sizes
SizeList PricePriceCart
50 ug$435.00Add to Cart

Smad3  (also known as Mothers against decapentaplegic homolog 3 Mothers against DPP homolog 3, Mad3, hMAD-3, JV15-2 or hSMAD3) is a 50 kDa member of a family of proteins that act as key mediators of TGF beta superfamily signaling in cell proliferation, differentiation and development. The Smad family is divided into three subclasses: receptor regulated Smads, activin/TGF beta receptor regulated (Smad2 and 3) or BMP receptor regulated (Smad 1, 5, and 8); the common partner, (Smad4) that functions via its interaction to the various Smads; and the inhibitory Smads, (Smad6 and 7). Activated Smad3 oligomerizes with Smad4 upon TGF beta stimulation and translocates as a complex into the nucleus, allowing its binding to DNA and transcription factors. Phosphorylation of the two TGF beta dependent serines 423 and 425 in the C terminus of Smad3 is critical for Smad3 transcriptional activity and TGF beta signaling.

Images

Paraffin-embedded tissue sections of human skin stained with anti-Smad3.

Paraffin-embedded tissue sections of human skin stained with anti-Smad3.