Blocking Buffer is intended to block non-specific background in fixed cells and tissues stained by using immunocytochemistry (ICC) and immunohistochemistry (IHC) applications. This blocking buffer is used to pre-incubate samples before adding primary antibodies. The buffer can be used on specimens fixed with different fixatives including, but not limited to, buffered formalin, formaldehyde, alcohols, acetone and chloroform.
Pre-incubation in Blocking Buffer allows eliminating non-specific background staining and enhancing signal-to-noise ratio of specific immunoreactivity. This buffer is compatible with frozen and paraffin-embedded tissue sections.
Typical application of the Cell & Tissue Blocking Buffer is as follows:
1. Pre-incubate samples with Blocking Buffer (30-100 uL per tissue section or cytosmear) for 10-30 min at room temperature;
2. Discard Blocking Buffer (it is not necessary to rinse samples with PBS, TBS and other wash buffers after discarding the Blocking Buffer);
3. Add primary antibodies diluted with Neuromics Antibody Dilution Buffer (SF40011) and incubate specimens for a specified period of time.