Product Details
Catalog Number: CH22104
Applications: ICC, WB, IHC
Type: Chicken IgY
Dilutions: ICC 1:100,000; IHC 1:100,000 WB 1:100,000
Immunogen: Bovine intermediate filaments from spinal cords
Storage: Antibody can also be aliquotted and stored frozen at -20° C to -70° C in a manual defrost freezer for six months without detectable loss of activity. The antibody can be stored at 2° - 8° C for 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles
Format A: liquid
Species Reactivity: Human, Mouse, Rat, Primate
Downloads: Datasheet (pdf)
Product Sizes
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50 ul$275.00Add to Cart

Neurofilaments can be defined as the intermediate or 10nm filaments found in specifically in neuronal cells. In the electron microscope neurofilaments appears as 10nm diameter fibres of indeterminate length which generally have fine wispy protrusions from their sides. They are found particularly abundantly in axons of large projection neurons. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons, and are biochemically very stable.

Some studies have shown that levels of neurofilament heavy and neurofilament light are elevated in patients with Alzheimer's disease, frontotemporal lobe dementia, and vascular dementia. 

Images

Shows rat mixed neuron/glial cultures stained with mouse monoclonal antibody to NF-L clone 7D1 (green) and chicken antibody to NF-H. This antibody binds primarily to the phosphorylated axonal forms of NF-H, in contrast to the NF-L antibody which stains both axonal and dendritic/perikaryal neurofilaments. The NF-L antibody therefore reveals a prominent cell body in green, while the surrounding axonal profiles are orange, since the are bound by both NF-L and the chicken NF-H antibody. Blue is a DNA stain.

Shows rat mixed neuron/glial cultures stained with mouse monoclonal antibody to NF-L clone 7D1 (green) and chicken antibody to NF-H. This antibody binds primarily to the phosphorylated axonal forms of NF-H, in contrast to the NF-L antibody which stains both axonal and dendritic/perikaryal neurofilaments. The NF-L antibody therefore reveals a prominent cell body in green, while the surrounding axonal profiles are orange, since the are bound by both NF-L and the chicken NF-H antibody. Blue is a DNA stain.