Images

View of mixed neuron/glial cultures stained with chicken polyclonal NF-L (red) and phosphorylated NF-H. Since both proteins are found in neurofilaments, the red and green patterns overlap, so that neurofilaments containing NF-L and phosphorylated NF-H appear yellowish. In contrast neurofilaments containing only NF-L appear red. Protocol on datasheet.

View of mixed neuron/glial cultures stained with chicken polyclonal NF-L (red) and phosphorylated NF-H. Since both proteins are found in neurofilaments, the red and green patterns overlap, so that neurofilaments containing NF-L and phosphorylated NF-H appear yellowish. In contrast neurofilaments containing only NF-L appear red. Protocol on datasheet.

NF-L western blots of whole protein extracts of rat spinal cord (lane 1), brain stem (lane 2), cerebellum (lane 3) and cerebral cortex (lane 4). Neurofilaments are concentrated in large projection axons and therefore NF-L is a much more major component of spinal cord than cortical regions

NF-L western blots of whole protein extracts of rat spinal cord (lane 1), brain stem (lane 2), cerebellum (lane 3) and cerebral cortex (lane 4). Neurofilaments are concentrated in large projection axons and therefore NF-L is a much more major component of spinal cord than cortical regions

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