Product Details
Catalog Number: MO22159
Applications: ICC, WB, IHC, IF
Type: Mouse IgG
Immunogen: Full length recombinant human protein aurora A expressed in and purified from E. coli
Format A: Affinity Purified
Format B: liquid
Species Reactivity: Human, Mouse, Rat, Chicken, Porcine, Equine
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$295.00Add to Cart

Left: Western blot analysis of MO22159 in HeLa cells. Blot of HeLa cells treated with 100ng/ml nocodazole for 18 hours was probed with MO22159. Nocodazole is a microtubule depolymerizing agent which induces cells to halt at the G2/M phase and also induces Aurora A expression. The MO22159 monoclonal binds strongly to a band at about 46 kDa, which is Aurora A and also shows binding to a band at 38 kDa. Right: Blots of recombinant human Aurora A, B and C were probed with MCA-3H1, which binds to both Aurora A and B. We conclude that the 38 kDa band seen in the HeLa extract is Aurora B. This is also consisted with the immunocytochemical staining of both centrosomes and midbodies seen on HeLa cells.