c-Fos is part of the Fos gene family; which consists of 4 members: FOS, FOSb, FOSL1 and FOSL2. Fos family genes are associated with JUN proteins and other basic leucine ipper (bZIP) domain proteins to create a variety of AP-1 (activator protein-1) complexes. c-Fos is considered to be an immediate-early gene because expression is normally low but rapidly and transiently in response to a wide array of stimuli including serum, growth factors, tumor promoters, cytokines, and UV radiation. It plays an important role in many cellular functions, and is over-expressed in variety of cancers.
Left: MO22162 staining (green) in HeLa cells, which were treated with serum-starvation for 36 hours, followed by 2 hours, 20% FBS stimulation (bottom panel), or PBS treatment (top panel). Green c-Fos staining only localizes in the nuclei of stimulated cells, but not in un-stimulated cells. Cells are counter-stained with our chicken polyclonal antibody against vimentin, CH22108 in red). Blue shows DAPI staining of nucleus．Middle: Mouse brain section (45 μM; fixed by transcardial perfusion with 4% paraformaldehyde) labeled with MO22162 using a standard HRP-DAB (horseradish peroxidase-3,3’-diaminobenzidine) staining technique. Cells expressing c-Fos show dark color in nucleus. Right: Mouse cortical section labeled with MO22162 (red) and our rabbit polyclonal anti Fox3/NeuN (RA22113) antibody (green) using immuno-fluorescent confocal-microscopy. Neurons positive for c-Fos and Fox3/NeuN appear to be yellow. Inset shows an enlarged image of MCA-2H2 staining. Nuclei are labeled with Dapi (blue)
Top panel: Western blot analysis of c-Fos expression in HeLa cells using MO22162. Lane 1: HeLa cells were serum-starved for 36 hours. 2: Serum-starved HeLa cells were stimulated with 20% FBS (fetal bovine serum) for 2 hours. MO22162 recognizes bands in the range of 50-65 kDa, which represent multiple forms of c-Fos. Serum starvation attenuates c-Fos expression, while 20% FBS strongly stimulates c-Fos expression. Bottom panel: Blot was stripped and probed with our monoclonal antibody against GAPDH, MO25038 used as loading control.
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