Product Details
Catalog Number: MO22183
Applications: ICC, WB, IHC, IF
Type: Mouse IgG
Dilutions: Western Blot: 1:5,000. Immunofluorescence/Immunocytochemistry: 1:500.
Immunogen: Recombinant construct, amino acids 317-630 of the human protein.
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Affinity Purified
Format B: liquid
Species Reactivity: Human, Mouse, Rat
Entrez: 10763
UniProt: P48681
Downloads: Datasheet (pdf)
Product Sizes
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100 ug$325.00Add to Cart

Nestin is a member of the class IV intermediate filament protein family which is expressed in neuroepithelial stem cells. The protein proved to be an unusual member of the intermediate filament family, containing an α-helical region homologous to that found in keratin and neurofilament subunits. The nestin protein has a very short non-helical N-terminal region followed by the α-helical region and a very long and repetitive C-terminal region. Nestin is expressed by radial glia and other types of dividing cells in the developing central and peripheral nervous systems and in developing muscle.

Images
Immunofluorescence Nestin

Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with mouse mAb to nestin, MO22183, dilution 1:500 in red, and costained with chicken pAb to MAP2 in green.

Immunofluorescence Nestin

Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with mouse mAb to nestin, MO22183, dilution 1:500 in red, and costained with chicken pAb to MAP2 in green.