- Delta Opioid Receptor
Delta Opioid Receptor 3-17 staining in rat dorsal horn. Data courtesy of Dr. Janet Keast, University of New South Wales.
A-D-Staining of Differentiated Stem Cells. D3 ES cells were maintained in serum/LIF/ME induced self-renewal conditions (undifferentiated cells, D3). Upon treatment with 1 µM RA they undergo differentiation (dD3). Cells were fixed and permeabilized. Treatments were followed by overnight incubation with polyclonal MOR-1 (C-terminus, 1:2500)
Eunhae Kim, Amy L. Clark, Alexi Kiss, Jason W. Hahn, Robin Wesselschmidt, Carmine J. Coscia and Mariana M. Belcheva. (2006). μ- and κ-Opioids Induce the Differentiation of Embryonic Stem Cells to Neural Progenitors. The Journal of Biological Chemistry. 281, 33749-33760. doi: 10.1074/jbc.M603862200
- Mu Opioid Recetor - Rabbit
Double immunofluorescence labeling of VR1 C-terminus (TRPV1, GP14100) and Mu Opioid Receptor in the DRG and spinal cord.
Confocal images showing the effect of RTX on u opioid receptor- and TRPV1-immunoreactive DRG neurons and afferent terminals in the spinal cord. A: representative confocal images showing u opioid receptor (green) and TRPV1 (red) immunoreactivities in DRG neurons of one vehicle- and one RTX-treated rat. Scale bar, 40 um. B: confocal images showing u opioid receptor (green) and TRPV1 (red) immunoreactivities in afferent terminals in the spinal dorsal horn of 1 vehicle- and 1 RTX-treated rat. Scale bar, 80 um. Inset: high-magnification images (scale bar = 5 um) showing co-localization of u opioid receptor and TRPV1 immunoreactivity in the lamina I. Co-localization of the u opioid receptor and TRPV1 immunoreactivity is indicated in yellow when 2 images are digitally merged. All images are single confocal optical sections.
Shao-Rui Chen and Hui-Lin Pan. (2005). Loss of TRPV1-Expressing Sensory Neurons Reduces Spinal u Opioid Receptors But Paradoxically Potentiates Opioid Analgesia. J Neurophysiol 95: 3086 –3096. doi:10.1152/jn.01343.2005.
- More Mu Opioid Receptor
Confocal images showing distribution of µ opioid receptor and synaptophysin immunoreactivities in the CeA ( central nucleus of the amygdala). A, immunoreactivities of synaptophysin (a, red) and µ opioid receptor (b, green) in the CeA. c, merged images from a and b; colocalization of µ opioid receptors and synaptophysin is illustrated in yellow. B, confocal images showing spatial relationship of a biocytin-labeled CeA cell (a, blue) with synaptophysin (b, red) and µ opioid receptor (c, green) immunoreactivities. The presynaptic colocalization of the µ opioid receptor and synaptophysin is indicated by color change (yellow, d) in merged images of a, b, and c. C, another biocytin-labeled CeA cell showing a presynaptic location of the µ opioid receptor immunoreactivity. Images are in all cases single confocal optical sections.
Thomas F. Finnegan, Shao-Rui Chen and Hui-Lin Pan. (2005) Effect of the μ Opioid on Excitatory and Inhibitory Synaptic Inputs to Periaqueductal Gray-Projecting Neurons in the Amygdala. Journal of Pharmacology and Experimental Therapeutics 312 (2) 441-448; DOI: https://doi.org/10.1124/jpet.104.074633