The protein proved to be an unusual member of the intermediate filament family, containing an α-helical region homologous to that found in keratin and neurofilament subunits. The Human Genome Organization (HUGO) Gene Nomenclature Committee (HGNC) name for Nestin is NES.
Image: SH-SY5Y neuroblastoma cells were stained with Nestin (RA221025) at 1:5,000 (red) and CH22108 against vimentin (green) at 1: 10,000. Vimentin is main partner of nestin forming heterdimers and further polymerizing to form intermediate filaments. Co-localization of vimentin and nestin in cells was shown in yellow. Blue is DAPI staining of DNA.
Nestin is relatively poorly conserved in protein sequence across species boundaries, so that the mouse and human proteins have an overall identity of only 62%. As a result antibodies to the human protein often fail to recognize the rodent homologue and vice versa. Antibodies to nestin are widely used to identify neural stem cells. This antibody was raised in rabbit against recombinant human nestin from aa 315-630, which has only 55% identity to homologous mouse sequence. Nestin (RA22125) therefore recognizes nestin protein in human, but not in rodent.
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Name Catalog # Neurofilament alpha-internexin/NF66 CH22101 Vimentin CH22108 Nestin CH23001 Netrin-1 CH23002 Tuj 1 (Neuron-specific class III beta-tubulin) CH23005 Vimentin CH23010 Neuritin GT15022 Nucleostemin GT15050 Semaphorin 6a GT15056 Semaphorin 7A GT15076 Semaphorin 3C GT15077 Otx2 GT15095 SOX2 GT15098 Nestin GT15114 Musashi-1 GT15116 Notch2 GT15124 Activin RIIA GT15149 S100B GT15160 Netrin-4 GT15164 Semaphorin 6B GT15165 Jagged1 GT15171 Mash1 GT15216 Frizzled 4/CD344 GT15217 TROY/TNFRSF19 GT15223 NeuroD1 GT15231 Brg1 GT15244 TROY/TNFRSF19, Biotinylated GT15332B MSX1/HOX7 GT41015 PAX3 GT41025 Nestin MO15012 Tuj 1 (Neuron-specific class III beta-tubulin) MO15013 A2B5 MO15016 Semaphorin 6a MO15023 Otx2 MO15039 SOX2 MO15040 Semaphorin 7A MO15041 Mash1 MO15048 Musashi-1 MO15049 Nestin MO15056 CD4 MO20025 Doublecortin/DCX MO22113 GAP43 MO22118 Neurofilament alpha-internexin/NF66 MO25017 ATPase Na+/K+ transporting alpha 1 MO25023 Musashi-1 RA14128 AP-2 Alpha RA18023 Notch1 RA19069 Notch3 RA19070 Neurofilament alpha-internexin/NF66 RA22102 Laminin 111 RA22121 SOX2 RA25021 Laminin RA25038 GAP43 RA25086