Product Details
Catalog Number: RA25086
Applications: WB, IHC, FC
Type: Rabbit IgG
Immunogen: Synthetic peptide corresponding to amino acids 216-226 of rat/mouse GAP-43, (C)-KEDPEADQEHA coupled to maleimide-activated KLH.
Format A: Affinity Purified
Format B: liquid
Species Reactivity: Human, Mouse, Rat, Chicken
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$285.00Add to Cart

GAP-43 (growth associated protein 43 kDa; also known as neuromodulin) is a 24 kDa, neuron growth cone-associated member of the neuromodulin family. Apparently due to its tertiary structure, it runs anomalously at 43 kDa on SDS-polyacrylamide gel. Rat GAP-43 is a 226 amino acid (aa) protein that contains an N-terminal lipid binding motif, an 11 aa IQ domain (IQ:Ile-Gln-X-Ser-etc) (aa 38 - 48), and a C-terminus with four potential serine phosphorylation sites. The molecule may utilize the IQ domain to bind calmodulin. Protein kinase C-mediated phosphorylation of the Serine at 41 within this site inhibits this interaction. Unphosphorylated, calmodulin-bound GAP-43 is always associated with neurite retraction, while S41 phosphorylated GAP-43 stabilizes actin filaments and promotes growth cone extension.

Image: GAP43 staining of region CA1-CA4 of the hippocampus. Note intenese staining of cell processes.

Note: This antibody is useful for Western analysis where it recognizes a band at 43 kDa. GAP43 is a proven marker for developing and regenerating neurons, and to a lesser extent, reactive glial cells. It is used widely to specifically label injured neurons and to score neuronal regeneration.

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