The Strep-tag® purification system is based on the highly selective binding of engineered streptavidin, called Strep-Tactin, to Strep-tag II fusion proteins. This technology allows one-step purification of almost any recombinant protein under physiological conditions, thus preserving its bioactivity. The Strep-tag system can be used to purify Strep-tag II proteins from any expression system including baculovirus, mammalian cells, yeast, and bacteria. For vectors for bacterial expression. Cloning and expression kits and vectors.
Simple one-step purification from crude lysate to 99% pure protein under physiological conditions possible.
High and selective binding affinity and high capacity due to special Strep-Tactin resins
Resins for gravity flow, low pressure or HPLC applications
Co-purification of non-covalently bound ligands, thus, protein-protein interaction studies are possible
Column regeneration and activity status is visualized by color change.
- Strep-tag® Detection Kits, Antibodies, and Conjugates
These kit and conjugates are designed for For chemiluminescence detection of Strep-tag proteins on Western blots. They do not require the use of secondary antibodies.
The HRP and AP Detection Kits include all buffers and reagents required for blocking, washing and the chromogenic reaction on a total of 1000 cm2 membrane.
Name Catalog # Type Species Applications Size Price BioLock Biotin blocking solution 2-0205-050 Blocking Buffer Not Applicable 50 ml $215.00 Biotin Blocking Buffer 2-0501-002 Blocking Buffer Not Applicable WB 2 ml $99.00 Strep-Tactin AP Conjugate 2-1503-001 Chromogenic Detection Step-Tactin Proteins WB 0.5 ml $175.00 Strep-Tactin HRP Conjugate 2-1502-001 Chromogenic Detection Step-Tactin Proteins WB 0.5 ml $175.00 Strep-Tactin® coated microplates 2-1501-001 Chromogenic Detection Step-Tactin Proteins E, Fluoro-Imaging 1X96 Well Plate $99.00 Strep-Tactin® coated microplates 2-1501-050 Mouse IgG Step-Tactin Proteins E, BSC-CM5 5X96 Well Plates $319.00 StrepMAB-Classic 2-1507-001 Mouse IgG Step-Tactin Proteins E, IF, FACS 100 ug $275.00 StrepMAB-Classic HRP conjugate 2-1509-001 Mouse IgG Step-Tactin Proteins WB 75 ul $359.00 StrepMAB-Immo 2-1517-001 Mouse IgG Step-Tactin Proteins E, BSC-CM5 100 ug $459.00
- Strep-Tactin®XT Superflow® high capacity
High affinity meets high capacity!
- Highest affinity available among affinity purification systems
- Higher protein yields compared to all other Strep-Tactin® resins
- Elution of concentrated protein due to small elution volumes
- Cost efficent: small price for more protein
Name Catalog # Type Species Applications Size Price 10x Buffer BXT; Strep-Tactin®XT Elution Buffer 2-1042-025 Purification 25 ml $64.00 Gravity flow Strep-Tactin®XT Superflow® high capacity column 2-4032-001 Column Purification 1 ml $119.00 Gravity flow Strep-Tactin®XT Superflow® high capacity column 2-4033-001 Column Protein Purification 5 ml $355.00 Gravity flow Strep-Tactin®XT Superflow® high capacity mini-column 2-4031-005 Column Protein Purification 5 x 0.2 ml $259.00 Strep-Tactin®XT Superflow® high capacity 50% suspension 2-4030-002 Protein Purification 4 ml $155.00 Strep-Tactin®XT Superflow® high capacity 50% suspension 2-4030-010 Protein Purification 20 ml $573.00 Strep-Tactin®XT Superflow® high capacity cartridge 2-4025-001 Purification 1 x 1 ml $129.00
- Strep-Tactin® XT Columns and Suspensions
The newly engineered Strep-Tactin®XT has an improved binding affinity to Twin-Strep-tag® and Strep-tag®II and is more stable compared to Strep-Tactin®. Strep-Tactin®XT now enables new applications in the field of high throughput screening, batch purification, purification using denaturing conditions and protein interaction studies. It also provides higher protein yields compared to Strep-Tactin®. Simultaneously, Strep-Tactin®XT preserves all benefits of Strep-Tactin® for protein purification, e.g. high purity (> 95 %), physiological buffer conditions, mild elution, regeneration of the resin for re-use.
Strep-Tactin XT:Twin-Srep-tag is the most versatile affinity purification system and can be used for all kind of applications.
Name Catalog # Type Species Applications Size Price Gravity flow Strep-Tactin® XT Superflow® column 2-4012-001 Column Protein Purification 1 x 1 ml $129.00 Gravity flow Strep-Tactin® XT Superflow® column 2-4013-001 Column Protein Purification 1 x 5 ml $339.00 Gravity flow Strep-Tactin® XT Superflow® column 2-4014-001 Column Protein Purification 1 x 10 ml $609.00 MagStrep "type3" XT beads 5% suspension 2-4090-002 Not Applicable 2 ml $275.00 Strep-Tactin® XT Superflow® 50% suspension 2-4010-002 Column Suspensions Protein Purification 4 ml $169.00 Strep-Tactin® XT Superflow® 50% suspension 2-4010-010 Column Suspensions Protein Purification, FPLC 20 ml $409.00 Strep-Tactin® XT Superflow® 50% suspension 2-4010-025 Column Suspensions Protein Purification, FPLC 50 ml $977.00
- Strep-Tactin® Columns and Suspensions
Five Strep-Tactin resin versions are available which differ in their properties and applications. While Strep-Tactin Sepharose® is preferentially used for gravity flow chromatography, Strep-Tactin® Superflow®, Strep-Tactin® Superflow® high capacity and Strep-Tactin MacroPrep® (specifications) can also be used for low pressure or FPLC applications, and Strep-Tactin POROS® (20 and 50) for FPLC and HPLC chromatography. Each matrix exhibits different non-specific binding properties.
In cases where unsatisfactory results are obtained with one matrix due to unspecific background, it is advisable to switch to one of the other matrices. In addition, Superflow is especially suited for increased flow rates and is also useful for the purification of large protein complexes. Please note, that the Strep-Tactin resins are optimized for column affinity chromatography as opposed to batch purification (Strep-tactin columns-resins comparisons table).
- Strep-tag® Buffers and Reagents
Lysis, Elution, Regeneration, Blocking and Washing Buffers
Name Catalog # Type Species Applications Size Price 10x Buffer BE Strep-Tactin® Elution Buffer with Biotin 2-1019-025 Wash Buffer Not Applicable 25 ml $65.00 Anhydrotetracycline 2-0401-001 Lysis Buffer Not Applicable 50 mg $235.00 Anhydrotetracycline 2-0401-002 Cloning Not Applicable 25 mg $125.00 GFP-Strep-tag control protein 2-1006-005 500 ug $109.00 Strep-tag II Desthiobiotin elution reagent 2-1000-025 Elution Buffer Not Applicable Protein Purification 25 ml $79.00 Strep-tag II Peptide 2-1018-002 Peptide Gravity Flow 1.8 mg $95.00 Strep-tag II Peptide 2-1018-009 Peptide Gravity Flow 5X1.8 mg $435.00 Strep-tag protein purification buffer set 2-1002-001 Not Applicable Protein Purification 1 Buffer Set $99.00 Strep-tag regeneration buffer with HABA 2-1002-100 Regeneration Buffer Not Applicable 100 ml $65.00 Strep-tag washing buffer 2-1003-100 Wash Buffer Not Applicable 100 ml $45.00