n-FectTM is a cationic lipid that has been emperically formulated for nervous system applications. n-Fect has a lower tocicity profile for primary neural cells than most cationic lipids.
Cell lines (see figure): n-Fect has worked well on neural and glial cells with a high transfection efficency.
Primary Neurons: Like most cationic lipids, the transfection effecency for most of our customers in primary neurons has been fairly low. The main observed advantage of n-Fect compared with other cationic lipid products is its low toxicity. At the recommended transfection ratios, neurons remain healthy. The observed transfection efficency in primary neurons has been approximately 10% - but has been variable from lab to lab. We therefore offer this kit in a trial size to test in your unique cell culture conditions.
n-Fect has been use for plasmid transfection in Experimental Crescentic Glomerulonephritis. See n-Fect: Publications
NF30150: 1.5 mls -75-300 Transfections
NF30750: 5 x 1.5 mls - 375-1500 Transfections
- Product Reviews
- Product Publications
1. Amy N. Sussman, Tong Sun, Ronald M. Krofft, and Raghu V. Durvasula. (2009). Accelerates Disease Progression in Experimental Crescentic Glomerulonephritis. Am. J. Pathol, 174: 1827 - 1836; doi: 10.2353/ajpath.2009.080464
2. Hidetaka Sadanari, Junji Tanaka, Zhuan Li, Rie Yamada, Keiko Matsubara and Tsugiya Murayama. (2009). Proteasome inhibitor differentially regulates expression of the major immediate early genes of human cytomegalovirus in human central nervous system-derived cell lines. Virus Research, doi: 10.1016/j.virusres.2009.01.010.
3. King-Ho Cheung, Diana Shineman, Marioly Müller, César Cárdenas, Lijuan Mei1, Jun Yang, Taisuke Tomita, Takeshi Iwatsubo, Virginia M.-Y. Lee, and J. Kevin Foskett. (2008). Mechanism of Ca2+ Disruption in Alzheimer's Disease by Presenilin Regulation of InsP3 Receptor Channel Gating. Neuron, doi: 10.1016/j.neuron.2008.04.015
4. Griffin SV, Hiromura K, Pippin J, Petermann AT, Blonski MJ, Krofft R, Takahashi S, Kulkarni AB, Shankland SJ. (2004). Cyclin-Dependent Kinase 5 Is a Regulator of Podocyte Differentiation, Proliferation, and Morphology. Am J Pathol. 165:1175-1185; doi: 10.1016/S0002-9440(10)63378-0
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