n-FectTM is a cationic lipid that has been emperically formulated for nervous system applications. n-Fect has a lower tocicity profile for primary neural cells than most cationic lipids.
Cell lines (see figure): n-Fect has worked well on neural and glial cells with a high transfection efficency.
Primary Neurons: Like most cationic lipids, the transfection effecency for most of our customers in primary neurons has been fairly low. The main observed advantage of n-Fect compared with other cationic lipid products is its low toxicity. At the recommended transfection ratios, neurons remain healthy. The observed transfection efficency in primary neurons has been approximately 10% - but has been variable from lab to lab. We therefore offer this kit in a trial size to test in your unique cell culture conditions.
n-Fect has been use for plasmid transfection in Experimental Crescentic Glomerulonephritis. See n-Fect: Publications
NF30150: 1.5 mls -75-300 Transfections
NF30750: 5 x 1.5 mls - 375-1500 Transfections
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