The pASG-IBA23 vector allows the expression of Strep-/GST-tag-fusion-proteins in E.coli. The vector carries the inducible tetracycline promoter/operator for the regulated expression of proteins, the Strep-tag® for C-terminal and the GST-tag for N-terminal fusion to the recombinant protein as well as the Ampicillin Resistance cassette. It can be used with any E. coli strain because the tet-promoter works independently from the genetic background of E.coli. The additional PreScission protease cleavage site allows the removal of the GST-tag.
The expressed recombinant protein will be localized in the cytoplasm.
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