The pASG-IBA167 vector allows the expression of Twin-Strep®-/FLAG-tag-fusion-proteins in E.coli. The vector carries the inducible tetracycline promoter/operator for the regulated expression of proteins, the Twin-Strep®-/FLAG- tags for N-terminal fusion to the recombinant protein as well as the Ampicillin Resistance cassette. It can be used with any E. coli strain because the tet-promoter works independently from the genetic background of E.coli[i/].
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