The pESG-IBA162 vector allows the expression of FLAG/Twin-Strep-tag®-fusion-proteins in Mammalia. The vector carries the Human cytomegalovirus (CMV) immediate-early promoter for high-level expression of proteins, the FLAG-tag for N-terminal and the Twin-Strep-tag® for C-terminal fusion to the recombinant protein as well as the Neomycin resistance gene for selection of stable cell lines. The expressed recombinant protein will be localized in the cytoplasm.
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