The pYSG-IBAwt1 vector allows the high-level expression of control proteins without tag in yeast. The vector carries the copper inducible promoter (CUP1) for controlled high-level expression, the URA3 auxotrophy marker for selection after transformation (do not use URA3 for selection during expression), the LEU2d auxotrophy marker for selection to increase plasmid copy number for expression (do not use LEU2d for selection after transformation). The expressed recombinant protein will be localized in the cytoplasm.
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