Fab-TACS® - Traceless Affinity Cell Selection - is a positive cell isolation approach based on our Strep-tag® technology. CD4 Fab-TACS® Agarose column Starter Kit allows label-free isolation of murine CD4+ T helper cells from splenocytes or other cell suspensions. CD4 is a co-receptor that associates with the T cell receptor and interacts with MHC class II molecules.
Strep-Tactin® TACS Agarose columns are filled with Strep-Tactin® coated agarose macrobeads developed for cell isolation. Fab fragments fused to a Twin-Strep-tag® (Fab-Streps) bind to the matrix. Target cells adhere to the matrix based on the exclusive binding of the Fab-Strep to a surface marker. Non-target cells are efficiently washed away. In a final step, the addition of biotin causes the elution of the target cells and the Fab-Streps. After elution, the Fab-Streps self-dissociate from the cell surfaces due to their reduced affinity. The label-free authentic target cells are now ready for further downstream applications.
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