CD19 Nano-TACS® Agarose column Starter Kit, mouse

Nano-TACS® - Traceless Affinity Cell Selection - is a positive cell isolation approach based on our Strep-tag® technology. CD19 Nano-TACS® Agarose column Starter Kit allows label-free isolation of murine B cells from splenocytes or other cell suspensions. CD19 is an adaptor protein that is involved in signaling pathways.

Strep-Tactin® TACS Agarose columns are filled with Strep-Tactin® coated agarose macrobeads developed for cell isolation. Twin-Strep-tagged nanobodies (Nano-Streps) bind to the matrix. Target cells adhere to the matrix based on the exclusive binding of the Nano-Strep to a surface marker. Non-target cells are efficiently washed away. In a final step, the addition of biotin causes the elution of the target cells and the Nano-Streps. After elution, the Nano-Streps self-dissociate from the cell surfaces due to their low affinity. The label-free authentic target cells are now ready for further downstream applications.

Advantages:

• Highly pure cells directly from whole blood
• Reversible binding to the target
• Highly viable, non-activated cells
• Label-free cells due to low affinity Fab fragments

Contents:

• Strep-Tactin® TACS Agarose Columns
• CD19 Fab-Strep, mouse
• 10x Buffer CI
• 100 mM Biotin stock solution
• TACS Column Adapter