Product Details
Catalog Number: 6-3319-002
Applications: Cell Assays
Type: Cell Isolation-Separation
Storage: Store all components at 2-8°C
Shipping: Ambient
Species Reactivity: Human
Downloads: Datasheet (pdf)
Product Sizes
SizeList PricePriceCart
1 Kit$158.50Add to Cart

Fab-TACS® is a traceless affinity exosome isolation approach based on our Strep-tag® technology. CD9 Fab-TACS® Exosome Agarose Column Starter Kit allows label-free isolation of human CD9+ exosomes from plasma, serum and cell culture supernatants. CD9 is a member of the tetraspanin family present on the surface of exosomes.

Strep-Tactin® TACS Agarose columns are filled with Strep-Tactin® coated agarose macrobeads developed for cell isolation. Fab fragments fused to a Twin-Strep-tag® (Fab-Streps) bind to the matrix. Exosomes adhere to the matrix based on the exclusive binding of the Fab-Strep to a surface marker. Non-target particles are efficiently washed away. In a final step, the addition of biotin causes the elution of the exosomes and the Fab-Streps. After elution, the Fab-Streps self-dissociate from the exosome surface due to their low affinity. The label-free, fully functional exosomes are now ready for further downstream applications.

Advantages:

  • Highly pure exosomes
  • Fully functional exosomes
  • Reversible binding to the target
  • Label-free exosomes due to low affinity Fab fragments
  • Quick and simple protocol without ultracentrifugation

The kit contains:

  • Strep-Tactin® TACS Agarose Columns
  • CD9 Fab-Strep, human
  • 100 mM Biotin stock solution
  • TACS Column Adapter
Images