Product Details
Catalog Number: 6-8000-207
Applications: Cell Assays
Type: Cell Isolation-Separation
Species Reactivity: Human
Product Sizes
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1 Kit$683.00Add to Cart

For isolation of CD25+ T cells from peripheral blood mononuclear cells. The kit includes all Fab Streptamer components for positive cell isolation with and for removal of Streptamers from isolated cells:

  • Strep-Tactin® Magnetic Microbeads* for Fab Streptamers: 2 vials for 5x108 cells each
  • CD25 Fab-Strep human for isolation of T-cells: 1 vial for 1x109 cells
  • D-Biotin stock solution for Streptamer® technology: 2x 1 ml
  • Buffer IS, Streptamer® Washing Buffer, 10x concentrated: 50 ml

*Microbeads are intended for use with a permanent magnet for manual cell isolation; we recommend our StrepMan magnet for 2 x 15 ml and 2 x 50 ml Falcon tubes. 

Isolation of human CD25+ T cells is performed by positive selection. PBMCs are labeled with magnetic CD25 Fab Streptamers. Labeled cells are isolated in a strong magnet (StrepMan, horizontal position) where they migrate toward the tube wall on the side of the magnet. This CD25 positive cell fraction is collected and cells are liberated from all labeling reagents by addition of biotin in a strong magnet (StrepMan, upright position). The magnetic Streptamers migrate toward the tube wall and the label-free cells remain in the supernatant. Biotin is removed by washing. The resulting cell preparation is highly enriched with CD25+ T cells with a purity of more than 90%. No depletion steps and no columns are needed.

Images

CD25+ T-Cell Isolation Process

CD25+ T-Cell Isolation Process