CD4 Fab-Strep is a recombinantly expressed low affinity Fab fragment fused to a Twin-Strep-tag®. Due to its low affinity for the target, an increase in avidity is required to ensure sufficient binding to the cell surface. Combining Fab-Streps with a Strep-Tactin® backbone leads to the formation of complexes that stably bind to cells.
Fab-Streps were developed for Traceless Affinity Cell Selection (Fab-TACS®), which is based on our Strep-tag® technology.
Depending on the Strep-Tactin® backbone, the following applications are possible:
Fluorescent cell staining and isolation: Fab-Streps are combined with fluorophore-conjugated Strep-Tactin®. The resulting complex is suitable for fluorescent cell staining and sorting.
Magnetic cell isolation: Fab-Streps are combined with Strep-Tactin® Magnetic Microbeads. The resulting complex is suitable for magnetic cell isolation.
Affinity chromatographic cell isolation: Fab-Streps can be loaded in Strep-Tactin® TACS Agarose Columns that are suitable for affinity chromatographic cell isolation.
By addition of biotin, the labeling reagents dissociate mildly and rapidly from the selected target cells in all applications, yielding minimally manipulated cells for unbiased further use.
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