Product Details
Catalog Number: MO22133
Applications: WB, IHC, IF
Type: Mouse IgG
Dilutions: Immunofluorescent: 1:500-1,000 Immunohistochemistry: 1:500-1,000 Western Blot: 1:1,000-2,000
Immunogen: Recombinant full length protein
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: liquid
Species Reactivity: Human, Mouse, Rat, Cat, Porcine
Entrez: 7447
UniProt: P62760
Downloads: Datasheet (pdf)
Product Sizes
SizeList PricePriceCart
100 ul of 1 mg/ml$275.00Add to Cart

Visinin-like protein 1 is a low molecular weight protein which is very abundant in the nervous system and is found only in neurons, though different neurons have different levels of expression. It is particularly concentrated in cerebellar Purkinje cells, and generally tends to be most abundant in neuronal perikarya and dendrites. The protein was discovered independently by several groups and is therefore also sometimes known as hippocalcin-like protein 3, HLP3, HPCAL3, HUVISL1, VLP-1, VILIP and VILIP-1. The protein belongs to the large superfamily of calmodulin and paravalbumin type proteins which function by binding Calcium ions. Calcium binding alters the conformation of these proteins and allow them to interact with other binding partners, the properties of which they may alter.

Images
Immunofluorescence

Confocal image of adult rat cerebellum stained with MO22133 antibody to visinin-like protein 1 in green, chicken polyclonal antibody to MAP2 in red, and DNA in blue. The MO22133 antibody reveals perikarya and synaptic regions in the neuron rich granular layer (bottom) and synapse rich molecular layer (top).

Immunofluorescence

Confocal image of adult rat cerebellum stained with MO22133 antibody to visinin-like protein 1 in green, chicken polyclonal antibody to MAP2 in red, and DNA in blue. The MO22133 antibody reveals perikarya and synaptic regions in the neuron rich granular layer (bottom) and synapse rich molecular layer (top).

Western Blot

Western blot analysis of different tissue lysates using mouse mAb to visinin-like protein 1 (VLP1) dilution 1:1,000, in green: [1] protein standard (red), [2] rat brain, [3] mouse brain, [4] pig hippocampus, and [5] cow cerebellum. The band at about 20kDa corresponds to the VLP1 protein.

Western Blot

Western blot analysis of different tissue lysates using mouse mAb to visinin-like protein 1 (VLP1) dilution 1:1,000, in green: [1] protein standard (red), [2] rat brain, [3] mouse brain, [4] pig hippocampus, and [5] cow cerebellum. The band at about 20kDa corresponds to the VLP1 protein.