Product Details
Catalog Number: MO22148
Applications: WB, IHC, IF
Type: Mouse IgG
Dilutions: Immunofluorescence/Immunocytochemistry: 1:5,000 Immunohistochemistry: 1:5,000 Western Blot: 1:5,000
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Affinity Purified
Format B: liquid
Species Reactivity: Human
Entrez: 11315
UniProt: Q99497
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$295.00Add to Cart

DJ-1, also known as Park7, is a cytoplasmic protein that belongs to the ThiJ/Pfp1/DJ1 superfamily that functions as protein chaperones, catalases, proteases and kinases. DJ-1 was originally cloned as an oncogene that cooperatively transforms cells together with H-ras. Mutations in the DJ-1 gene are associated with rare forms of autosomal recessive early-onset Parkinson's disease. DJ-1 may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. Augmenting DJ-1 activity might provide novel approaches to treating chronic neurodegenerative illnesses such as Parkinson's disease and acute damage such as stroke

Images

HeLa cells stained with MO22148 (green), and CH22108 chicken antibody to vimentin (red) and DNA (blue). MO22148 antibody reveals strong cytoplasmic staining for DJ-1 in human cells, but does not bind to rodent cells

HeLa cells stained with MO22148 (green), and CH22108 chicken antibody to vimentin (red) and DNA (blue). MO22148 antibody reveals strong cytoplasmic staining for DJ-1 in human cells, but does not bind to rodent cells

Blot of 20 µg rat brain lysate (lane 1) and 20 µg HeLa cell lysate (lane 2) was probed with MO22148 antibody to DJ-1at 1:5,000. Notes that the strong single band running at about 21kDa corresponds to DJ-1 in HeLa cells, while DJ-1 is barely seen in rat brain lysate. This antibody binds also to bovine DJ-1 on western blots but does not recognize rat or mouse DJ-1.

Blot of 20 µg rat brain lysate (lane 1) and 20 µg HeLa cell lysate (lane 2) was probed with MO22148 antibody to DJ-1at 1:5,000. Notes that the strong single band running at about 21kDa corresponds to DJ-1 in HeLa cells, while DJ-1 is barely seen in rat brain lysate. This antibody binds also to bovine DJ-1 on western blots but does not recognize rat or mouse DJ-1.