Product Details
Catalog Number: MO22193
Applications: ICC, WB, IHC, IF
Type: Mouse IgG
Dilutions: Immunohistochemistry: 1:1,000 Immunofluorescent: 1:1,000 Immunocytochemistry: 1:1,000 Western Blot: 1:5,000
Immunogen: C-terminal peptide of human NF-L protein, GEEEDTKESEEEEKKEESAGEEQVAKKKD with an N-terminal C for coupling to KLH
Storage: Store at 4°C short term. Store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Affinity Purified
Species Reactivity: Human, Mouse, Rat, Bovine, Porcine
Entrez: 4747
UniProt: P07196
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$325.00Add to Cart

Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H, though other filament proteins may be included also. The major function of neurofilaments is likely to control the diameter of large axons. NF-L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at 68-70kDa with some variability across species. Antibodies to NF-L like MCA-6H112 are useful for identifying neuronal cells and their processes in cell culture and sectioned material. NF-L antibody can also be useful for the visualization of neurofilament rich accumulations seen in many neurological diseases, such as Lou Gehrig’s disease (ALS), giant axon neuropathy, Charcot-Marie Tooth disease and others. Much interest has recently been focused on the detection of NF-L released from neurons into blood and CSF as a surrogate marker of primarily axonal loss in a variety of types of CNS injury and degeneration.

Images
Immunofluorescence

Immunofluorescent analysis of a rat frontal cortex section stained with mouse mouse monoclonal antibody to NF-L dilution 1:2,000 in green, and costained with rabbit polyclonal antibody to FOX3/NeuN dilution 1:2,000 in red.

Immunofluorescence

Immunofluorescent analysis of a rat frontal cortex section stained with mouse mouse monoclonal antibody to NF-L dilution 1:2,000 in green, and costained with rabbit polyclonal antibody to FOX3/NeuN dilution 1:2,000 in red.

Western Blot

Western blot analysis of spinal cord lysates from different species using mouse monoclonal antibody to NF-L dilution 1:5,000 in green: [1] protein standard (red), [2] rat, [3] mouse, [4] pig, and [5] cow spinal cord. Strong band at about 70kDa corresponds to the NF-L protein.

Western Blot

Western blot analysis of spinal cord lysates from different species using mouse monoclonal antibody to NF-L dilution 1:5,000 in green: [1] protein standard (red), [2] rat, [3] mouse, [4] pig, and [5] cow spinal cord. Strong band at about 70kDa corresponds to the NF-L protein.