Product Details
Catalog Number: MO25046
Applications: WB, IHC
Type: Mouse IgG
Immunogen: A peptide within residues 1-50 of the human protein. [Swiss-Prot# Q9GZT9]. Clone: 366G/76/3
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Affinity Purified
Format B: liquid
Species Reactivity: Human
Entrez: 54583
UniProt: Q9GZT9
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$325.00Add to Cart

HIF prolyl hydroxylase 2 is a prolyl hydroxylase that modifies HIF-alpha. Classic prolyl hydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIF is an intracellular protein and the HPH sites do not resemble those modifying collagen. HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. HPH is an essential component of the pathway through which cells sense oxygen. In the presence of oxygen, HPHs convert specific prolyl residues in HIF-alpha to hydroxyproline, leading to HIF-alpha destruction. Low oxygen levels, sensed at the cellular level, cause the HIF conversion to be reduced so that HIF is stable and there is increased angiogenesis. HPH-2, specifically, catalyzes the posttranslational formation of 4-hydroxyproline in HIF alpha proteins. It hydroxylates HIF-1 alpha at Pro(402) and Pro(564), and HIF-2 alpha. It targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex.    

Images

Western blot analysis in HeLa whole cell extracts using HIF Prolyl Hydroxylase 2 Antibody.

Western blot analysis in HeLa whole cell extracts using HIF Prolyl Hydroxylase 2 Antibody.