Product Details
Catalog Number: RA25066
Applications: WB
Type: Rabbit IgG
Immunogen: Genomic sequence made to an internal portion of the human protein (within residues 400-550). [Swiss-Prot# Q16665].
Storage: Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Affinity Purified
Format B: liquid
Species Reactivity: Human, Primate
Entrez: 3091
UniProt: Q16665
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$379.00Add to Cart

HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. It is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity. Therefore, the concentration of HIF-1 alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition. HIF hydroxylases provide insight into hypoxic cell responses, which may be used to help isolate therapeutic targets.

Images

Detection of HIF-1 alpha. Lane 1: COS7 CoCl treated cells Lane 2: COS7 untreated cells

Detection of HIF-1 alpha. Lane 1: COS7 CoCl treated cells Lane 2: COS7 untreated cells