Product Details
Catalog Number: CH22103
Applications: ICC, WB, IHC
Type: Chicken IgY
Dilutions: ICC 1:10,000; IHC 1:10,000; WB 1:100,000
Immunogen: Recombinant human projection domain sequence, amino acids 377-1505
Storage: Antibody can also be aliquotted and stored frozen at -20° C to -70° C in a manual defrost freezer for six months without detectable loss of activity. The antibody can be stored at 2° - 8° C for 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles
Format A: liquid
Species Reactivity: Human, Mouse, Rat, Primate
Downloads: Datasheet (pdf)
Product Sizes
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50 ul$299.00Add to Cart

Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein tau and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4, and MAP5. MAP2 is made up of two ~280kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70kDa on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule-binding peptide sequences, which are very similar to those found in the related microtubule binding protein tau.

MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. Antibodies to MAP2 are therefore excellent markers on neuronal cells, their perikarya and neuronal dendrites. In contrast, tau is found predominantly in neuronal axons.

Images
Confocal image of adult rat cerebellar cortex stained with VSNL1 (CH22132, green) and chicken polyclonal antibody to MAP2 (red) and DNA (blue)

Confocal image of adult rat cerebellar cortex stained with VSNL1 (CH22132, green) and chicken polyclonal antibody to MAP2 (red) and DNA (blue). VSNL1 antibody reveals synapses in the molecular layer (ML) strongly. Synaptic regions are also seen in the granule cell layer (GC). The perikarya of Purkinje cells (PC) are revealed with the MAP2 antibody (4). Little staining is seen in the white matter (WM).

Confocal image of adult rat cerebellar cortex stained with VSNL1 (CH22132, green) and chicken polyclonal antibody to MAP2 (red) and DNA (blue)

Confocal image of adult rat cerebellar cortex stained with VSNL1 (CH22132, green) and chicken polyclonal antibody to MAP2 (red) and DNA (blue). VSNL1 antibody reveals synapses in the molecular layer (ML) strongly. Synaptic regions are also seen in the granule cell layer (GC). The perikarya of Purkinje cells (PC) are revealed with the MAP2 antibody (4). Little staining is seen in the white matter (WM).

primary neuronal culture showing neuronal staining with MAP2 (green; Catalog # CH22103) and astrocytes with GFAP (red; Catalog # MO19063). Nuclei are counterstained blue.

primary neuronal culture showing neuronal staining with MAP2 (green; Catalog # CH22103) and astrocytes with GFAP (red; Catalog # MO19063). Nuclei are counterstained blue.

primary neuronal culture showing neuronal staining with MAP2 (green; Catalog # CH22103) and astrocytes with GFAP (red; Catalog # MO19063). Nuclei are counterstained blue.

primary neuronal culture showing neuronal staining with MAP2 (green; Catalog # CH22103) and astrocytes with GFAP (red; Catalog # MO19063). Nuclei are counterstained blue.