HBMECs were isolated from normal human brain tissue. Passage 2, cells are ship on dry ice in a vial or in proliferating culture with a confluency of >90 %*. ENDO-Growth medium containing 5% serum and growth supplements are recommended for culture. Cells have an average additional population doubling levels >15 when cultured.
Image: Human Brain Microvascular Endothelial Cells in Culture.
Characterization of the cells
HBMECs are negative for HIV-1, HBV, HCV, and mycoplasma.
Date of Expiration: Guaranteed for up to 6 months from date of shipping when stored in liquid nitrogen.
**cells are shipped at 90% confluence. However, confluence can be lost during shipping. Please allow for 1-4 days to reach 90% confluence again.
ENDO-Growth Media – MED001: Contains 475 ml of ENDO-Basal Media and 25 ml of ENDO-Growth Supplement combined. Which is freshly prepared for your convenience OR ENDO-Growth Kit – EGK001: Contains 475 of ENDO-Basal Media and 25 ml of ENDO-Growth Supplement separately to be mixed to make growth media
Smooth Coat Solution – SC300: Biocompatible complex of extracellular matrix binding solution