Product Details
Catalog Number: HEC02-T25
Applications: Cell Assays
Type: Primary Cells
Species Reactivity: Human
Downloads: Datasheet (pdf)
Product Sizes
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500,000+ Cells in T25 Flask$789.00Add to Cart

HBMECs were isolated from normal human brain tissue. Passage 2, cells are ship on dry ice in a vial or in proliferating culture with a confluency of >90 %*.  ENDO-Growth medium containing 5% serum and growth supplements are recommended for culture. Cells have an average additional population doubling levels >15 when cultured.

Image: Human Brain Microvascular Endothelial Cells in Culture.

Characterization of the cells
  • Cytoplasmic VWF / Factor VIII: >95% positive by immunofluorescence
  • Cytoplasmic uptake of Di-I-Ac-LDL: >95% positive by immunofluorescence
  • Cytoplasmic PECAM1 >95% positive by immunofluorescence 

HBMECs are negative for HIV-1, HBV, HCV, and mycoplasma.

**cells are shipped at 90% confluence. However, confluence can be lost during shipping. Please allow for 1-4 days to reach 90% confluence again.