Product Details
Catalog Number: HEC02-T25
Applications: Cell Assays
Type: Primary Cells
Species Reactivity: Human
Downloads: Datasheet (pdf)
Product Sizes
SizeList PricePriceCart
500,000+ Cells in T25 Flask$789.00Add to Cart

Culturing Protocol - T25 Flask 

HBMECs were isolated from normal human brain tissue. Passage 2, cells are ship on dry ice in a vial or in proliferating culture with a confluency of >90 %*.  ENDO-Growth medium containing 5% serum and growth supplements are recommended for culture. Cells have an average additional population doubling levels >15 when cultured.

Image: Human Brain Microvascular Endothelial Cells in Culture.

Characterization of the cells
  • Cytoplasmic VWF / Factor VIII: >95% positive by immunofluorescence
  • Cytoplasmic uptake of Di-I-Ac-LDL: >95% positive by immunofluorescence
  • Cytoplasmic PECAM1 >95% positive by immunofluorescence 

HBMECs are negative for HIV-1, HBV, HCV, and mycoplasma.

Date of Expiration: Guaranteed for up to 6 months from date of shipping when stored in liquid nitrogen.

**cells are shipped at 90% confluence. However, confluence can be lost during shipping. Please allow for 1-4 days to reach 90% confluence again. 

Recommended Products

ENDO-Growth Media – MED001Contains 475 ml of ENDO-Basal Media and 25 ml of ENDO-Growth Supplement combined. Which is freshly prepared for your convenience                                                                                                                OR                                                                                                                                                                                                                ENDO-Growth Kit – EGK001: Contains 475 of ENDO-Basal Media and 25 ml of ENDO-Growth Supplement separately to be mixed to make growth media

 Smooth Coat Solution – SC300 Biocompatible complex of extracellular matrix binding solution
OR
AlphaBioCoat Solution – AC001: Premium Smooth Coat Solution. Biocompatible complex of extracellular matrix binding solution with growth factors. Ideal for culturing cells from frozen.

Cell Detachment Solution – ADF001: Contains protease and collagenase activities in an isotonic, phosphate buffer solution with EDTA to detach primary cells and cell lines

1X Phosphate Buffer Solution - PBS300

 

Supporting Products
NameCatalog #
AlphaBioCoat SolutionAC001
Cell Detachment SolutionADF001
ENDO-Growth Media KitEGK001
ENDO-Growth SupplementsEGS001
Endothelial Cryopreserve MediaFM300
ENDO-Growth MediaMED001
ENDO Basal MediaMED002
1X Phosphate Buffer SolutionPBS300
Smooth Coat SolutionSC300
Images