Image: Western blots of HeLa cell crude extracts. Lane 16 was probed with HSP27, while lanes 14 and 15 were probed with two other monoclonals to HSP27 we generated at the same time. Note the strong clean bands at 27kDa. Lane 17 was probed with our new mouse monoclonal antibody to Lamin A/C, which binds two bands running at 74kDa and 65kDa. Lane 13 was probed with our monoclonal antibody to all six actin isotypes. Molecular weights of each protein are as indicated, and dots indicate the presence of major HeLa proteins. Protocol on Datasheet.