Product Details
Catalog Number: MO22151
Applications: ICC, WB, IHC, IF
Type: Mouse IgG
Format A: Affinity Purified
Format B: liquid
Species Reactivity: Human, Mouse, Rat, Bovine, Porcine
Downloads: Datasheet (pdf)
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100 ul$295.00Add to Cart

Splicing factor SF3B4, also known as SAP49, is a ubiquitously expressed splicing factor found in the nuclei of eukaryotic cells, although it migrates into the cytoplasm of dividing cells. It was originally identified by as the protein most efficiently UV cross-linked to the A, B and C spliceosomal complexes.

Image: Human HeLa cells stained with MO22151 (red), chicken antibody to vimentin (CH22108) (green) and DNA (blue, stained with DAPI). The MCA-3A1 antibody reveals strong granular nuclear staining which is a little different from the DNA stain and presumably reflects splicosomal complexes. The vimentin antibody stains the cytoplasmic intermediate filament network of the HeLa cells.

The protein contains two of the highly conserved RRM type RNA recognition motifs, each corresponding to a small ~70 amino acid structure, consisting of 4 beta strands and two alpha-helices. Proteins containing these proteins are believed to have a role in the regulation of mRNA splicing. The protein runs on SDS-PAGE gels at an apparent molecular weight of 49kDa. This protein is known as splicing factor 3b, subunit 4, 49kDa SAP49, spliceosome-associated protein (U2 snRNP), Hsh49 and MGC108282. Antibodies to this protein are good markers of nuclei. 

 

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