Product Details
Catalog Number: MO22156
Applications: ICC, WB, IHC, IF
Type: Mouse IgG
Format A: Affinity Purified
Format B: liquid
Species Reactivity: Human, Mouse, Rat, Chicken, Porcine, Equine
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$295.00Add to Cart

Aldolase A is generally considered to be a muscle enzyme. Northern analysis of cultured cells suggests that it is present in both neurons and glia. Aldolase C shares 81% amino acid identity with aldolase A and 70% identity with aldolase B.

Earlier studies using isozyme-specific antibodies report its location in gray matter astrocytes and cells of the pia mater. In situ hybridization of mouse central nervous system using isozyme-specific probes revealed that aldolase A and C are expressed in complementary cell types: aldolase A mRNA is found in neurons; aldolase C message is detected in astrocytes, some cells of the pia mater, and Purkinje cells. Aldolase C can in some situations be used as an astrocyte marker. However Purkinje cells of the cerebellum contain high levels of the enzyme, so the enzyme is not totally astrocyte specific.

Images

View of mixed neuron/glial cultures stained with MO22156 (red) and our chicken antibody to GFAP antibody (CH22102, green).

View of mixed neuron/glial cultures stained with MO22156 (red) and our chicken antibody to GFAP antibody (CH22102, green). MO22156 antibody reveals strong cytoplasmic staining in astrocytes. Blue is a DNA stain. Aldolase stains the astrocytes cell body and processes, whereas GFAP labels the intermediate filament of the cytoskeleton in subset of astrocytes.

View of mixed neuron/glial cultures stained with MO22156 (red) and our chicken antibody to GFAP antibody (CH22102, green).

View of mixed neuron/glial cultures stained with MO22156 (red) and our chicken antibody to GFAP antibody (CH22102, green). MO22156 antibody reveals strong cytoplasmic staining in astrocytes. Blue is a DNA stain. Aldolase stains the astrocytes cell body and processes, whereas GFAP labels the intermediate filament of the cytoskeleton in subset of astrocytes.