Product Details
Catalog Number: P10110
Type: Guinea Pig IgG
Format A: Whole serum
Format B: liquid
Downloads: Datasheet (pdf)
Product Sizes
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100 ug Blocking Peptide$95.00Add to Cart

Image: TRPV1 binds to the PI3K SH2 domains in a phosphotyrosine-independent manner. (A) In vitro interaction assay using TRPV1-FLAG from HEK293 cells (left) or recombinant N terminus of TRPV1 from bacteria with a FLAG epitope at its C-terminal end (right). FLAG-tagged TRPV1 protein was immobilized on anti-FLAG agarose beads, and recombinant GST fusion proteins corresponding to PI3K-p85{alpha} were tested for interaction. The bars in the cartoon represent the two independent clones of PI3K-p85ß identified in the yeast 2-hybrid assay. For the Western blot, equivalent amounts of input and bound proteins were probed with anti-GST antibody. (B) The recombinant N-terminal protein was not tyrosine phosphorylated. The recombinant FLAG-tagged protein was run in the left lane, and lysates from trkA-transfected HEK293 cells were run in the right lane. Duplicate gels were run, and Western blot analysis was then performed with either the anti-FLAG antibody (top) or the anti-phosphotyrosine antibody (bottom). (C) TRPV1 in HEK293 cells was not tyrosine phosphorylated. HEK293 cells expressing combinations of TRPV1, p75, and trkA, as indicated in the figure, were treated with either NGF or vehicle and then immunoprecipitated with anti-trkA (lanes 1–7) or anti-TRPV1 (lanes 8–13) antibodies. Lanes 1–7 were separated from lanes 8–13 and the membranes were probed with anti-trkA antibody and anti-TRPV1 antibody, respectively (top). Segments of the membrane were then reapposed for imaging. The membranes were then stripped and reprobed with anti-phosphotyrosine antibody (bottom). Published online Oct 30 2006. doi:10.1085/jgp.200609576
© The Rockefeller Press

  • Product Reviews
    Product Reviews
    Everything went smoothly! Product Name: proDynorphin (rat), Guinea Pig – (Cat# GP10110) http://bit.ly/Sw4RJ9 Organization: University of Texas - Austin
    simone - Jan 27, 2017 - Rating: 5.0
    Product shipped timely and in secured conditions to ensure antibody viability. We are currently optimizing a protocol with their guinea pig anti-prodynorphin primary antibody. I am happy to revisit Neuromics for future research needs. Product Name: proDynorphin (rat), Guinea Pig – (Cat# GP10110) http://bit.ly/Sw4RJ9 Organization: Temple University
    Steven J Simmons - Feb 05, 2016 - Rating: 5.0
    Good order service. Quick delivery. Easy order from website. Product Name: proDynorphin (rat), Guinea Pig – (Cat# GP10110) http://bit.ly/Sw4RJ9 Organization: OHSU
    Rubing Xing - Oct 02, 2015 - Rating: 5.0
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