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- Catherine Reed-McBain, Rithvik V. Turaga, Seth R.T. Zima, Janmesh Patel, Anderson Weber Faletti Cunha, Jason Mixdorf, Lauren E Wehner, Jonathan W. Engle, Reinier Hernandez, Stevens K Rehen, Helena L Borges, and Jose M Ayuso. (2025). Non-Destructive Luminescence and PET Imaging to Monitor Tissue Microenvironment in Microphysiological Systems During Brain Metastasis Using Dissociated Cerebral Organoids. Biofabrication. doi: 10.1088/1758-5090/ade1fb
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Images
Immunofluorescence analysis of HeLa cells costained with rabbit pAb to vimentin, RA22124, dilution 1:5,000, in green, and mouse mAb to Actin dilution 1:500, in red. Blue is DAPI staining of nuclear DNA. The vimentin antibody stains the 10nm or intermediate filament network of the cytoskeleton. The antibody to actin labels the submembranous actin-rich cytoskeleton, stress fibers, and bundles of actin associated with cell adhesion sites
Immunofluorescence analysis of HeLa cells costained with rabbit pAb to vimentin, RA22124, dilution 1:5,000, in green, and mouse mAb to Actin dilution 1:500, in red. Blue is DAPI staining of nuclear DNA. The vimentin antibody stains the 10nm or intermediate filament network of the cytoskeleton. The antibody to actin labels the submembranous actin-rich cytoskeleton, stress fibers, and bundles of actin associated with cell adhesion sites
Western blot analysis of whole cell lysates using rabbit pAb to vimentin, RA22124, dilution 1:5,000, in green. [1] protein standard (red), [2] HeLa, [3] SH-SY5Y, [4] HEK293, [5] NIH-3T3 cells. Strong band corresponds to vimentin protein with apparent SDS-PAGE molecular weight of ~55kDa
Western blot analysis of whole cell lysates using rabbit pAb to vimentin, RA22124, dilution 1:5,000, in green. [1] protein standard (red), [2] HeLa, [3] SH-SY5Y, [4] HEK293, [5] NIH-3T3 cells. Strong band corresponds to vimentin protein with apparent SDS-PAGE molecular weight of ~55kDa