Product Details
Catalog Number: RA24288
Applications: WB, IHC
Type: Rabbit IgG
Dilutions: IHC 1:1000-1:2000
Immunogen: multiple antigenic peptide of an N-terminal synthetic sequence corresponding to amino acids 22-41 of rat 5HT2A receptor
Storage: Store at 4°C. Avoid freeze-thaw cycles.
Shipping: Refrigerate (Polar Packs)
Format A: Affinity Purified
Format B: liquid
Species Reactivity: Rat
Entrez: 3356
UniProt: P28223
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$399.00Add to Cart

The affinity purified histochemical antibody for 5HT2A receptor was generated in rabbit against a multiple antigenic peptide of an N-terminal synthetic sequence corresponding to amino acids 22-41 of rat 5HT2A receptor.

The antibody produces 4+ (maximum) immunolabeling of 5HT2A receptor at dilutions of 1/300 - 1/500 using biotin-streptavidin/HRP technique with free-floating methods on rat cortex, amygdala and hippocampus. The addition of intensifying reagents such as nickel ammonium sulfate to the chromogen solution allows further dilution to approximately 1/1,000 or more. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended. Immunolabeling is completely abolished by preadsorption with synthetic rat 5HT2A receptor (22-41). Immunolabeling of Western blot revealed a single band of approximately between 55 and 75 kDA. For Western Blot Protocol See: DOI:10.1124/jpet.111.18378.

Images
5HT2AR staining of rat cerebral cortex.

5HT2AR staining of rat cerebral cortex.

5HT2AR staining of rat cerebral cortex.

5HT2AR staining of rat cerebral cortex.

Mouse frontal cortical neuron cultures (p1 pups, day 4 culture stained-Neuromicsantibody: 5-HT2AR at 1:100 48 hours. MAP2 Ab: 1:20,000, 24 h. Details in Schmid, Raehal, and Bohn; 2008; PNAS

Mouse frontal cortical neuron cultures (p1 pups, day 4 culture stained-Neuromicsantibody: 5-HT2AR at 1:100 48 hours. MAP2 Ab: 1:20,000, 24 h. Details in Schmid, Raehal, and Bohn; 2008; PNAS

Mouse frontal cortical neuron cultures (p1 pups, day 4 culture stained-Neuromicsantibody: 5-HT2AR at 1:100 48 hours. MAP2 Ab: 1:20,000, 24 h. Details in Schmid, Raehal, and Bohn; 2008; PNAS

Mouse frontal cortical neuron cultures (p1 pups, day 4 culture stained-Neuromicsantibody: 5-HT2AR at 1:100 48 hours. MAP2 Ab: 1:20,000, 24 h. Details in Schmid, Raehal, and Bohn; 2008; PNAS