Product Details
Catalog Number: SF40005
Applications: Fluoro-Imaging
Type: Blocking Reagent
Downloads: Datasheet (pdf)
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100 ml$89.00Add to Cart

FluoMute™ ready-to-use reagent to reduce autofluorescence in cells and tissue. Just incubate fixed cells of tissue sections with FluoMute™ for 30-60 min at room temperature, rinse with PBS and continue with immunofluorescence ICC or/and IHC protocols. Treatment with FluoMute™ does not affect cell morphology and the integrity of tissue antigens to be detected with primary antibodies (see bottom image). FluoMute™ is compatible with paraffin-embedded and frozen tissue sections, stem cells, lymphocytes and mammalian cell lines of different origin.

Application Notes:

 

  • Paraffin-embedded tissue sections: Use FluoMute after cleared in zylene/ethanol, and right be adding primary antibodies to cells and tissue sections.
  • If heat induced antigen retrieval is performed: FluoMute should be used after finishing retrieval procedure, and right be adding primary antibody to cells and tissue sections. 
  • Frozen tissue sections: FluoMute is used as the very last step after doing blocking, permeabilization and other steps, and right before adding primary antibodies. 

 


  • Product Reviews
    Product Reviews
    I ordered FluoMute, Autofluorescence Blocking Agent – (Cat# SF40005). Came super fast. Great company and products! I have used them over 5 years! Product Name: FluoMute, Autofluorescence Blocking Agent – (Cat# SF40005) http://bit.ly/2q1rFmo Organization: University of Wisconsin
    Kellie - May 23, 2017 - Rating: 5.0
    Amazing customer service, friendly and quick to respond to questions. Product Name: FluoMute, Autofluorescence Blocking Agent – (Cat# SF40005) http://bit.ly/2q1rFmo Organization: University of Toronto
    Lucia - May 17, 2017 - Rating: 5.0
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