The Strep-tag® purification system is based on the highly selective binding of engineered streptavidin, called Strep-Tactin, to Strep-tag II fusion proteins. This technology allows one-step purification of almost any recombinant protein under physiological conditions, thus preserving its bioactivity. The Strep-tag system can be used to purify Strep-tag II proteins from any expression system including baculovirus, mammalian cells, yeast, and bacteria. For vectors for bacterial expression. Cloning and expression kits and vectors.
-
Simple one-step purification from crude lysate to 99% pure protein under physiological conditions possible.
-
High and selective binding affinity and high capacity due to special Strep-Tactin resins
-
Resins for gravity flow, low pressure or HPLC applications
-
Co-purification of non-covalently bound ligands, thus, protein-protein interaction studies are possible
-
Column regeneration and activity status is visualized by color change.
- Strep-tag® Detection Kits, Antibodies, and Conjugates
These kit and conjugates are designed for For chemiluminescence detection of Strep-tag proteins on Western blots. They do not require the use of secondary antibodies.
The HRP and AP Detection Kits include all buffers and reagents required for blocking, washing and the chromogenic reaction on a total of 1000 cm2 membrane.
Name Catalog # Type Species Applications Size Price Strep-Tactin AP Conjugate 2-1503-001 Chromogenic Detection Step-Tactin Proteins WB 0.5 ml $169.00 Strep-Tactin HRP Conjugate 2-1502-001 Chromogenic Detection Step-Tactin Proteins WB 0.5 ml $169.00 Strep-Tactin® coated microplates 2-1501-001 Chromogenic Detection Step-Tactin Proteins E, Fluoro-Imaging 1X96 Well Plate $68.00 Strep-Tactin®XT DY-488 2-1562-050 Protein 5 x 50 µg $1,355.00 Strep-Tactin®XT DY-488 2-1562-050 Protein 50 µg $286.00 Strep-Tactin®XT DY-549 2-1565-050 Protein 5 x 50 µg $1,355.00 Strep-Tactin®XT DY-549 2-1565-050 Protein 50 µg $286.00 Strep-Tactin®XT DY-649 2-1568-050 Protein 5 x 50 µg $1,355.00 Strep-Tactin®XT DY-649 2-1568-050 Protein 50 µg $286.00 StrepMAB-Classic 2-1507-001 Mouse IgG Step-Tactin Proteins E, IF, FACS 100 ug $276.00 StrepMAB-Classic DY-488 2-1563-050 Mouse IgG 5 x 50 µg $1,720.00 StrepMAB-Classic DY-488 2-1563-050 Mouse IgG 50 µg $362.00 StrepMAB-Classic DY-549 2-1566-050 Mouse IgG 5 x 50 µg $1,720.00 StrepMAB-Classic DY-549 2-1566-050 Mouse IgG 50 µg $362.00 StrepMAB-Classic DY-649 2-1569-050 Mouse IgG 5 x 50 µg $1,720.00 StrepMAB-Classic DY-649 2-1569-050 Mouse IgG 50 µg $362.00 StrepMAB-Classic HRP conjugate 2-1509-001 Mouse IgG Step-Tactin Proteins WB 75 ul $349.00 StrepMAB-Immo 2-1517-001 Mouse IgG Step-Tactin Proteins E, BSC-CM5 100 ug $466.00 StrepMAB-Immo DY-488 2-1564-050 Mouse IgG 5 x 50 µg $1,890.00 StrepMAB-Immo DY-488 2-1564-050 Mouse IgG 50 µg $398.00 StrepMAB-Immo DY-549 2-1567-050 Mouse IgG 5 x 50 µg $1,890.00 StrepMAB-Immo DY-549 2-1567-050 Mouse IgG 50 µg $398.00 StrepMAB-Immo DY-649 2-1570-050 Mouse IgG 5 x 50 µg $1,890.00 StrepMAB-Immo DY-649 2-1570-050 Mouse IgG 50 µg $398.00 Twin-Strep-tag® Capture Kit 2-4370-000 1 kit $685.00 Twin-Strep-tag® Capture Maxi Kit 2-4370-010 1 Kit $1,431.00 - Strep-tag® Buffers and Reagents
Lysis, Elution, Regeneration, Blocking and Washing Buffers
Name Catalog # Type Species Applications Size Price Anhydrotetracycline 2-0401-002 Cloning Not Applicable 25 mg $125.00 BioLock Biotin blocking solution 2-0205-050 Blocking Buffer Not Applicable 50 ml $233.00 Desthiobiotin 2-1000-002 1 g $226.00 Desthiobiotin 2-1000-005 Not Applicable 5 g $996.00 Desthiobiotin 2-1000-005 Not Applicable 5 x 5 g $4,870.00 GFP-Strep-tag control protein 2-1006-005 500 ug $127.00 GFP-Twin-Strep-tag control protein 2-1007-105 Protein 500 ul $127.00 Strep-tag II Desthiobiotin elution reagent 2-1000-025 Elution Buffer Not Applicable Protein Purification 25 ml $72.00 Strep-tag II Peptide 2-1018-002 Peptide Gravity Flow 1.8 mg $100.00 Strep-tag II Peptide 2-1018-002 Peptide Gravity Flow 5 x 1.8 mg $450.00 Strep-tag protein purification buffer set 2-1002-001 Not Applicable Protein Purification 1 Buffer Set $113.00 Strep-tag regeneration buffer with HABA 2-1002-100 Regeneration Buffer Not Applicable 100 ml $59.00 Strep-tag washing buffer 2-1003-100 Wash Buffer Not Applicable 100 ml $50.00 WET FRED For 1 ml Gravity flow columns 2-0911-001 Not Applicable Cell Culturing System 1 Set $148.00 WET FRED For 5-10 ml Gravity flow columns 2-0910-001 Not Applicable Cell Culturing System 1 Set $167.00 - Strep-Tactin®XT Superflow® high capacity
High affinity meets high capacity!
Key benefits:
- Highest affinity available among affinity purification systems
- Higher protein yields compared to all other Strep-Tactin® resins
- Elution of concentrated protein due to small elution volumes
- Cost efficent: small price for more protein
- Strep-Tactin® XT Columns and Suspensions
The newly engineered Strep-Tactin®XT has an improved binding affinity to Twin-Strep-tag® and Strep-tag®II and is more stable compared to Strep-Tactin®. Strep-Tactin®XT now enables new applications in the field of high throughput screening, batch purification, purification using denaturing conditions and protein interaction studies. It also provides higher protein yields compared to Strep-Tactin®. Simultaneously, Strep-Tactin®XT preserves all benefits of Strep-Tactin® for protein purification, e.g. high purity (> 95 %), physiological buffer conditions, mild elution, regeneration of the resin for re-use.
Strep-Tactin XT:Twin-Srep-tag is the most versatile affinity purification system and can be used for all kind of applications.
Name Catalog # Type Species Applications Size Price Magnetic Separator 2-1602-000 Not Applicable 1 Piece $301.00 MagStrep "type3" XT beads 5% suspension 2-4090-002 Not Applicable 2 ml $234.00 MagStrep Strep-Tactin®XT Beads 2-5090-002 2 ml $234.00 MagStrep Strep-Tactin®XT Beads 2-5090-002 5 x 2 ml $845.00 MagStrep Strep-Tactin®XT Beads 2-5090-010 10 ml $812.00 MagStrep Strep-Tactin®XT Beads 2-5090-010 10 x 10 ml $7,300.00 Strep-Tactin®XT 4Flow® column 2-5012-001 Protein Purification 1 ml $101.00 Strep-Tactin®XT 4Flow® column 2-5013-001 Purification 5 ml $302.00 Strep-Tactin®XT 4Flow® column 2-5014-001 Protein Purification 10 ml $554.00 Strep-Tactin®XT 4Flow® column 2-5011-005 Protein Purification 5 x 0.2 ml $233.00 Strep-Tactin®XT 4Flow® Starter Kit 2-5998-000 Protein Purification 1 kit $228.00 Strep-Tactin®XT BLI Coupling Kit 2-4380-000 1 Kit $860.00 - Strep-Tactin® Columns and Suspensions
Five Strep-Tactin resin versions are available which differ in their properties and applications. While Strep-Tactin Sepharose® is preferentially used for gravity flow chromatography, Strep-Tactin® Superflow®, Strep-Tactin® Superflow® high capacity and Strep-Tactin MacroPrep® can also be used for low pressure or FPLC applications, and Strep-Tactin POROS® (20 and 50) for FPLC and HPLC chromatography. Each matrix exhibits different non-specific binding properties.
In cases where unsatisfactory results are obtained with one matrix due to unspecific background, it is advisable to switch to one of the other matrices. In addition, Superflow is especially suited for increased flow rates and is also useful for the purification of large protein complexes. Please note, that the Strep-Tactin resins are optimized for column affinity chromatography as opposed to batch purification.
- Strep-Tactin® Buffers and Reagents
Name Catalog # Type Species Applications Size Price Avidin 2-0204-050 Protein 50 mg $340.00 BioLock Biotin blocking solution 2-0205-250 Blocking Buffer Not Applicable 250 ml $941.00 Buffer XT-R 2-1045-250 Regeneration Buffer Not Applicable 250ml $66.00 MagStrep "type3" XT beads 5% suspension 2-4090-010 10 ml $812.00 MagStrep "type3" XT beads 5% suspension 2-4090-010 10 x 10 ml $7,300.00 Strep-Tactin® 2-1204-001 1 mg $139.00 Strep-Tactin® 2-1204-005 5 mg $416.00 Strep-Tactin® MacroPrep® resin 2-1505-010 Protein Purification 20 ml $518.00 Strep-Tactin® MacroPrep® resin 2-1505-010 Protein Purification 5 x 20 ml $2,400.00 Strep-Tactin® XT coated microplate 2-5101-001 1 Plate $70.00 Strep-Tactin®XT Buffer Set 2-1043-000 Not Applicable 1 Set $134.00 WET FRED For 1 ml Gravity flow columns 2-0911-001 Not Applicable Cell Culturing System 1 Set $148.00 WET FRED For 5-10 ml Gravity flow columns 2-0910-001 Not Applicable Cell Culturing System 1 Set $167.00