By Western blot mouse anti-ABCA1 (MO13101; clone HJ1) recognizes rat (left image), mouse (right image) and to a lesser extent human (left image) ABCA1.
David Holtzman and colleagues at Washington University in St. Louis have recently published several papers in the Journal of Biological Chemistry demonstrating the role of ABCA1 in ApoE regulation and that an increase in ABCA1 may cause a decrease in amyloid deposition. The data presented suggest in the papers below suggests a central role for ABCA1 in Alzheimer's biology, leading the authors to speculate ABCA1 as a potential drug target.
Wahrle SE, Jiang H, Parsadanian M, Legleiter J, Han X, Fryer JD, Kowalewski T, and Holtzman DM (2004). ABCA1 is Required for Normal Central Nervous System ApoE Levels and for Lipidation of Astrocyte-secreted apoE. J Biol. Chem. 279(39):40987.
Wahrle SE, Jiang H, Parsadanian M, Hartman RE, Bales KR, Paul SM and Holtzman DM (2005). Deletion of Abca1 Increases Abeta Deposition in the PDAPP Transgenic Mouse Model of Alzheimer Disease. J. Biol. Chem. 280(52):43236.
Please note this antibody also works for WB in Rabbit Hippocampal Tissue.
Image and Figure: Western blot analysis of hepatic ABCA1 expression in 16 hours fasted HSL-null and wildtype mice on ND or HFD for 27-29 weeks (n = 2 each group). Equal amounts of protein were loaded onto the gel for comparison of HSL-null and wildtype mice.The results on the graph are expressed as a percentage of control, that is the corresponding wildtype for a given group i.e. ND fed, ND fasted, HFD fed or HFD fasted group.
- Product Reviews
- Product Publications
- Michele Cavalera, Ulrika Axling, Catarina Rippe, Karl Swärd, and Cecilia Holm. (2017). Dietary rose hip exerts anti-atherosclerotic effects and increases nitric oxide-mediated dilation in ApoE-null mice. The Journal of Nutritional Biochemistry, doi: 10.1016/j.jnutbio.2017.02.017
- Prasanthi JRP, Huls A, Thomasson S, Thompson A, Schommer E, Ghribi O. (2009). Differential effects of 24-hydroxycholesterol and 27-hydroxycholesterol on beta-amyloid precursor protein levels and processing in human neuroblastoma SH-SY5Y cells. Molecular Neurodegeneration, 4:1. doi: 10.1186/1750-1326-4-1
- Celine Fernandez, Marie Lindholm, Morten Krogh, Stéphanie Lucas, Sara Larsson, Peter Osmark, Karin Berger, Jan Boren, Barbara A Fielding, Keith N. Frayn and Cecilia Holm. (2008). Disturbed cholesterol homeostasis in hormone-sensitive lipase null mice. Am J Physiol Endocrinol Metab, doi: 10.1152/ajpendo.90206.2008
- Related Products