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- Khanh Van Doan, Le Trung Tran, Dong Joo Yang, Thu Thi Anh Ha, Thi Dang Mai, Seul Ki Kim, Ronald A. DePinho, Dong-Min Shin, Yun-Hee Choi, and Ki Woo Kim. (2023). Astrocytic FoxO1 in the Hypothalamus Regulates Metabolic Homeostasis by Coordinating Neuropeptide Y Neuron Activity. Glia, 1–18. doi: 10.1002/glia.24448
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Images
Left: MO22162 staining (green) in HeLa cells, which were treated with serum-starvation for 36 hours, followed by 2 hours, 20% FBS stimulation (bottom panel), or PBS treatment (top panel). Middle: Mouse brain section labeled with MO22162 using a standard HRP-DAB staining technique Right: Mouse cortical section labeled with MO22162 (red) and our rabbit polyclonal anti Fox3/NeuN (RA22113) antibody (green) using immuno-fluorescent confocal-microscopy
Left: MO22162 staining (green) in HeLa cells, which were treated with serum-starvation for 36 hours, followed by 2 hours, 20% FBS stimulation (bottom panel), or PBS treatment (top panel). Middle: Mouse brain section labeled with MO22162 using a standard HRP-DAB staining technique Right: Mouse cortical section labeled with MO22162 (red) and our rabbit polyclonal anti Fox3/NeuN (RA22113) antibody (green) using immuno-fluorescent confocal-microscopy
Top panel: Western blot analysis of c-Fos expression in HeLa cells using MO22162. Lane 1: HeLa cells were serum-starved for 36 hours. 2: Serum-starved HeLa cells were stimulated with 20% FBS (fetal bovine serum) for 2 hours. MO22162 recognizes bands in the range of 50-65 kDa. Bottom panel: Blot was stripped and probed with our monoclonal antibody against GAPDH, MO25038 used as loading control.
Top panel: Western blot analysis of c-Fos expression in HeLa cells using MO22162. Lane 1: HeLa cells were serum-starved for 36 hours. 2: Serum-starved HeLa cells were stimulated with 20% FBS (fetal bovine serum) for 2 hours. MO22162 recognizes bands in the range of 50-65 kDa. Bottom panel: Blot was stripped and probed with our monoclonal antibody against GAPDH, MO25038 used as loading control.