Product Details
Catalog Number: RA22105
Applications: ICC, WB, IHC
Type: Rabbit IgG
Dilutions: ICC 1:100; IHC 1:100; WB 1:1,000
Immunogen: Recombinant fusion protein containing the extreme C-terminal segment of rat NF-M
Storage: Antibody can also be aliquotted and stored frozen at -20° C to -70° C in a manual defrost freezer for six months without detectable loss of activity. The antibody can be stored at 2° - 8° C for 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles
Format A: Whole serum
Format B: liquid
Species Reactivity: Human, Mouse, Rat, Chicken, Cat
Downloads: Datasheet (pdf)
Product Sizes
SizeList PricePriceCart
100 ul$275.00Add to Cart

Neurofilaments can be defined as the intermediate or 10nm filaments found in specifically in neuronal cells. In the electron microscope neurofilaments appears as 10nm diameter fibres of indeterminate length which generally have fine wispy protrusions from their sides. They are found particularly abundantly in axons of large projection neurons. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons, and are biochemically very stable.

Image: Western blot of whole rat cerebellum homogenate stained with NF-M, at dilution of 1:20,000. A prominent band running with an apparent SDS-PAGE molecular weight of ~145kDa corresponds to rodent NF-M. Human, cow and bovine NF-M run a little slower, at about 160kDa. Protocol on data-sheet.